Zoratti M, Petronilli V
FEBS Lett. 1985 Dec 2;193(2):276-82. doi: 10.1016/0014-5793(85)80168-x.
The relationship between rate of ATP synthesis and transmembrane electrochemical proton gradient has been determined in rat liver mitochondria oxidizing succinate, using the respiratory inhibitor malonate or the uncoupler FCCP to decrease delta microH progressively. As previously reported [(1982) Eur. J. Biochem. 126, 443-451] two different relationships are obtained depending on the method used. Evidence is presented that this result is not due to underestimation of the delta microH maintained by fast-respiring mitochondria, as recently suggested [(1985) FEBS Lett. 181, 323-327].
利用呼吸抑制剂丙二酸或解偶联剂FCCP逐步降低ΔμH，测定了在氧化琥珀酸的大鼠肝线粒体中ATP合成速率与跨膜电化学质子梯度之间的关系。如先前报道[(1982)欧洲生物化学杂志126, 443 - 451]，根据所使用的方法可得到两种不同的关系。有证据表明，该结果并非如最近所提出的[(1985)欧洲生物化学学会联合会快报181, 323 - 327]那样，是由于对快速呼吸线粒体维持的ΔμH估计不足所致。
