非磷酸化线粒体中的呼吸控制由质子泄漏和呼吸链共同承担。
Control of respiration in non-phosphorylating mitochondria is shared between the proton leak and the respiratory chain.
作者信息
Brand M D, Hafner R P, Brown G C
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1988 Oct 15;255(2):535-9.
Abstract
We measured the relationship between rate of respiration and membrane potential in isolated mitochondria titrated with malonate (to inhibit the electron transport chain) or with uncoupler (to increase the proton conductance of the inner membrane). We used the flux control summation and connectivity theorems of metabolic control theory to calculate the control over non-phosphorylating respiration exerted by the respiratory chain (and associated reactions) and by the leak of protons across the inner membrane. At 37 degrees C the flux control coefficient of the leak over respiration was 0.66; the flux control coefficient of the chain over respiration was 0.34. At 25 degrees C the values were 0.75 and 0.25 respectively. We argue that the basis for previous conclusions that all the control is exerted by the proton leak under similar conditions is invalid.
摘要
我们测定了用丙二酸（抑制电子传递链）或解偶联剂（增加内膜质子传导性）滴定的分离线粒体中呼吸速率与膜电位之间的关系。我们运用代谢控制理论的通量控制总和及连通性定理，来计算呼吸链（及相关反应）和质子跨内膜泄漏对非磷酸化呼吸的控制作用。在37℃时，质子泄漏对呼吸的通量控制系数为0.66；呼吸链对呼吸的通量控制系数为0.34。在25℃时，相应的值分别为0.75和0.25。我们认为，先前关于在类似条件下所有控制均由质子泄漏施加的结论的依据是无效的。
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