Graversen Katrine B, Bjarnov-Nicolau Bella, Kløve Sigri, Halajová Krístina, Andersen Sandra B
Center for Evolutionary Hologenomics, Globe Institute, University of Copenhagen, Denmark.
bioRxiv. 2025 May 5:2025.04.30.650706. doi: 10.1101/2025.04.30.650706.
The stomach bacterium is estimated to infect half of the world's population, and the health implications are affected by the age at infection. Neonatal infection of mice is a relevant model to investigate metabolic and immunological effects. We performed an explorative study at the dynamic first month of life, to compare the composition of the gastrointestinal tract microbiome and stomach gene expression of mice neonatally infected with with that of uninfected mice. We found that was present only in the stomach, and that loads increase with age from one week after infection and onwards, especially after weaning. Stomach and colon microbiome composition was strikingly similar between sites at the same sampling time, but changed significantly over one week, with increased diversity at both sites. Despite that the relative abundance of in the stomach was low and never exceeded 3%, the composition and alpha diversity of the gastrointestinal microbiome was significantly affected by infection. In a pathway enrichment analysis we found that stomach gene expression related to the extracellular matrix, muscle contraction, and metabolism was affected by infection. Expression of these key processes was, in infected mice, shifted away from that of control mice, towards that of all mice sampled the subsequent week, which we speculate represents accelerated development in infected mice.
据估计，这种胃部细菌感染了全球一半的人口，其对健康的影响受感染年龄的影响。新生小鼠感染是研究代谢和免疫效应的相关模型。我们在生命的第一个动态月进行了一项探索性研究，以比较新生感染小鼠与未感染小鼠的胃肠道微生物群组成和胃部基因表达。我们发现该细菌仅存在于胃部，并且从感染后一周及之后，尤其是断奶后，其载量随年龄增加。在相同采样时间，胃部和结肠的微生物群组成在不同部位之间惊人地相似，但在一周内发生了显著变化，两个部位的多样性均增加。尽管胃部中该细菌的相对丰度较低，从未超过3%，但胃肠道微生物群的组成和α多样性仍受到感染的显著影响。在一项通路富集分析中，我们发现与细胞外基质、肌肉收缩和代谢相关的胃部基因表达受感染影响。在感染小鼠中，这些关键过程的表达从对照小鼠的表达转向随后一周采样的所有小鼠的表达，我们推测这代表感染小鼠的加速发育。
