Sugihartono Titong, Fauzia Kartika Afrida, Miftahussurur Muhammad, Waskito Langgeng Agung, Rejeki Purwo Sri, I'tishom Reny, Alfaray Ricky Indra, Doohan Dalla, Amalia Rizki, Savitri Camilia Metadea Aji, Rezkitha Yudith Annisa Ayu, Akada Junko, Matsumoto Takashi, Yamaoka Yoshio
Division of Gastroenterology-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Jalan Mayjend Prof. Dr. Moestopo No. 6-8, Surabaya, 60286, Indonesia.
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita, 879-5593, Japan.
Gut Pathog. 2022 Sep 13;14(1):38. doi: 10.1186/s13099-022-00510-3.
We evaluated the microbiota in the stomach of Gastroesophageal Reflux Disease (GERD) patients. We compared Erosive Reflux Disease (ERD) to gastritis and Non-erosive Reflux Disease (NERD) subjects by 16S rRNA approach on gastric biopsy specimens. A total of 197 subjects were included consisting of gastritis (68; 34.52%), ERD (55; 27.92%), and NERD (74; 37.56%). After quality filtering, 187 samples were included for OTU analysis using Qiime2.
We observed a significant difference in alpha diversity (Shannon and Simpson indexes were P = 0.0016 and P = 0.017, respectively). A significant decrease in alpha diversity index was observed in NERD with Helicobacter pylori (H. pylori)-positive subjects than in gastritis (Simpson index P = 0.022; Shannon index P = 0.029), indicating a significant influence of H. pylori on the diversity in the stomach despite the diseases. In H. pylori-negative samples, alpha diversity measurement by the abundance coverage estimates (ACE) and Fisher Test revealed that ERD had significantly lower richness than gastritis and NERD groups (P = 0.00012 and P = 0.00043, respectively). Anaerobacillus sp. could only be found in ERD patients by LEFse analysis.
The presence of ERD could alter microbiome diversity. A negative correlation between H. pylori and ERD is shown in this microbiome study but not in NERD.
我们评估了胃食管反流病（GERD）患者胃内的微生物群。我们通过对胃活检标本采用16S rRNA方法，将糜烂性反流病（ERD）与胃炎和非糜烂性反流病（NERD）受试者进行了比较。总共纳入了197名受试者，包括胃炎患者（68名；34.52%）、ERD患者（55名；27.92%）和NERD患者（74名；37.56%）。经过质量过滤后，使用Qiime2对187个样本进行OTU分析。
我们观察到α多样性存在显著差异（香农指数和辛普森指数的P值分别为0.0016和0.017）。与胃炎相比，幽门螺杆菌（H. pylori）阳性的NERD受试者的α多样性指数显著降低（辛普森指数P = 0.022；香农指数P = 0.029），这表明尽管存在疾病，H. pylori对胃内的多样性仍有显著影响。在H. pylori阴性样本中，通过丰度覆盖估计（ACE）和费舍尔检验进行的α多样性测量显示，ERD的丰富度显著低于胃炎和NERD组（P值分别为0.00012和0.00043）。通过LEFse分析，仅在ERD患者中发现了厌氧芽孢杆菌属。
ERD的存在可能会改变微生物群的多样性。在这项微生物群研究中显示H. pylori与ERD呈负相关，但在NERD中未发现这种相关性。
