Ludwig S R, Pohlman R F, Vieira J, Smith A G, Messing J
Gene. 1985;38(1-3):131-8. doi: 10.1016/0378-1119(85)90211-2.
The 1913-bp maize mitochondrial (mt) plasmid was isolated from a suspension culture of a Black Mexican Sweet maize strain, cloned into M13mp vectors, and sequenced by a unidirectional progressive deletion method. The 1.9-kb extrachromosomal double-stranded circular DNA plasmid was found to contain regions of sequence which in other systems are known to be part of origins of replication (ori). This plasmid could be used as a carrier for chimeric genes and a molecular probe for replication.
1913bp的玉米线粒体（mt）质粒是从墨西哥黑甜玉米品系的悬浮培养物中分离出来的，克隆到M13mp载体中，并通过单向逐步缺失法进行测序。发现这个1.9kb的染色体外双链环状DNA质粒含有在其他系统中已知为复制起点（ori）一部分的序列区域。该质粒可作为嵌合基因的载体和复制的分子探针。
