Sisk D R, Kuwabara T
Graefes Arch Clin Exp Ophthalmol. 1985;223(5):250-8. doi: 10.1007/BF02153655.
Monosodium L-glutamate is known to cause intracellular swelling, necrosis, and disappearance of most inner retinal neurons, with concomitant thinning of inner retinal layers within hours after subcutaneous injection into neonatal rodents. A similar process can be observed in adult rat retina after intravitreal glutamate injection. To better describe and compare this process with that reported after systemic application, adult Sprague-Dawley rat eyes were intravitreally injected with 1 mumol monosodium L-glutamate and the retinas studied by LM and EM over a 2-month period. Results demonstrated that adult rat retina experienced severe degenerative changes which progressed in two stages: an initial stage of massive intracellular swelling and a second stage of necrosis and cell loss. Degeneration involved ganglion and inner nuclear layer cells. Inner retinal thickness decreased concurrently. By 2 months post-injection, degenerative changes in rod spherules and some loss of photoreceptor nuclei were observed. Both time course and severity of the lesion differed from that described in prior studies after systemic treatment.
众所周知，将L-谷氨酸钠皮下注射到新生啮齿动物体内数小时后，会导致细胞内肿胀、坏死以及大多数视网膜内层神经元消失，同时视网膜内层变薄。玻璃体内注射谷氨酸后，成年大鼠视网膜也可观察到类似过程。为了更好地描述这一过程并与全身应用后的情况进行比较，将1 μmol L-谷氨酸钠玻璃体内注射到成年Sprague-Dawley大鼠眼中，并在2个月内通过光镜和电镜对视网膜进行研究。结果表明，成年大鼠视网膜经历了严重的退行性变化，该变化分两个阶段进行：第一阶段为大量细胞内肿胀，第二阶段为坏死和细胞丢失。退变涉及神经节细胞和内核层细胞。视网膜内层厚度同时降低。注射后2个月，观察到杆状小体的退行性变化和一些光感受器细胞核的丢失。病变的时间进程和严重程度均与先前全身治疗研究中描述的不同。
