Keramari Stergiani, Xagorari Angeliki, Sotiropoulos Damianos, Sakellari Ioanna, Savopoulos Christos, Chlichlia Katerina, Fidani Liana, Kaiafa Georgia
First Propaedeutic Department of Internal Medicine, University General Hospital of Thessaloniki AHEPA, Thessaloniki, GRC.
Department of Haematology and Public Cord Blood Bank, General Hospital of Papanikolaou, Thessaloniki, GRC.
Cureus. 2025 Jun 13;17(6):e85944. doi: 10.7759/cureus.85944. eCollection 2025 Jun.
Umbilical cord blood (UCB) units are an alternative valuable source for allogeneic hematopoietic stem cells (allo-HSCT). Patients undergoing allo-HSCT remain at high risk for complications and mortality due to viral infections such as human adenovirus (HAdV). HAdV, a double-stranded DNA virus, has been detected in the full-term placenta, raising concerns about vertical transmission. This study aims to examine the presence of HAdV in UCB samples to improve the safety of transplantation procedures. Methods: A total of 98 UCB plasma samples were assayed to detect the presence of HAdV DNA using real-time polymerase chain reaction (PCR) technology Results: Of the 98 UCB plasma samples analyzed for HAdV, three tested positive (3.06%). In control testing for prevalent pathogens, 67.3% of the samples were Cytomegalovirus (CMV) IgG-positive (indicating past infection) and 4.2% were CMV IgM-positive (recent or active infection). All the samples were negative for human T-lymphotropic virus I-II (HTLV_I-II), hepatitis C virus (HCV), hepatitis B surface antigen (HBsAg), and human immunodeficiency virus I-II (HIV_I-II).
The detection of HAdV in a subset of UCB samples highlights the need to consider molecular screening for HAdV as part of routine safety evaluations in allogeneic UCB transplantation.
脐带血（UCB）单位是异基因造血干细胞（allo-HSCT）的另一种有价值的来源。接受allo-HSCT的患者由于人类腺病毒（HAdV）等病毒感染，仍面临并发症和死亡的高风险。HAdV是一种双链DNA病毒，已在足月胎盘中检测到，这引发了对垂直传播的担忧。本研究旨在检测UCB样本中HAdV的存在，以提高移植程序的安全性。方法：使用实时聚合酶链反应（PCR）技术对总共98份UCB血浆样本进行检测，以检测HAdV DNA的存在。结果：在分析HAdV的98份UCB血浆样本中，三份检测呈阳性（3.06%）。在常见病原体的对照检测中，67.3%的样本巨细胞病毒（CMV）IgG呈阳性（表明既往感染），4.2%的样本CMV IgM呈阳性（近期或活动性感染）。所有样本的人类嗜T淋巴细胞病毒I-II型（HTLV_I-II）、丙型肝炎病毒（HCV）、乙型肝炎表面抗原（HBsAg）和人类免疫缺陷病毒I-II型（HIV_I-II）均为阴性。结论：在一部分UCB样本中检测到HAdV，这凸显了在异基因UCB移植的常规安全性评估中，需要考虑对HAdV进行分子筛查。
