Urbánková Lucie, Kašpárková Věra, Dad'ová Eliška, Srnec Adam, Humpolíček Petr
Department of Fat, Surfactant and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, Zlín 760 01, Czech Republic.
Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T.G.Masaryka 5555, Zlin 760 01, Czech Republic.
ACS Omega. 2025 Jun 27;10(26):27722-27734. doi: 10.1021/acsomega.4c09731. eCollection 2025 Jul 8.
This study investigated freeze-dried Pickering emulsions stabilized by a combination of cellulose nanocrystals (CNCs) and sodium caseinate (CAS), with encapsulated curcumin. Our approach focused on the order of CNC/CAS addition and its influence on emulsion properties, along with the effect of three different cryoprotectants (sucrose, d-mannitol, and d-glucose) on the preservation of emulsion droplets. In the study, controlled release of curcumin from freeze-dried emulsions was achieved, attributed to the composition of the stabilizing layer and the cryoprotectant used. The emulsions were partially able to withstand freeze-drying and could be redispersed to samples with droplets bigger than those observed before freeze-drying. The best-preserved droplets came from emulsions stabilized first by CNC particles and then by CAS addition and protected with d-glucose. Transdermal penetration studies revealed that curcumin was mainly present on the skin's surface and at the , with limited penetration into deeper skin layers. Nevertheless, the samples showed outstanding antioxidant activity and no cytotoxicity effects, demonstrating their promising potential to positively influence the healing of the skin.
本研究考察了由纤维素纳米晶体（CNC）和酪蛋白酸钠（CAS）组合稳定、包封姜黄素的冻干Pickering乳液。我们的方法重点关注CNC/CAS添加顺序及其对乳液性质的影响，以及三种不同冷冻保护剂（蔗糖、D-甘露醇和D-葡萄糖）对乳液滴保存的影响。在该研究中，实现了姜黄素从冻干乳液中的控释，这归因于稳定层的组成和所使用的冷冻保护剂。乳液能够部分耐受冻干，并且可以重新分散成液滴比冻干前观察到的更大的样品。保存最好的液滴来自首先由CNC颗粒稳定、然后添加CAS并用D-葡萄糖保护的乳液。透皮渗透研究表明，姜黄素主要存在于皮肤表面和[此处原文缺失部分内容]，向皮肤深层的渗透有限。然而，这些样品显示出出色的抗氧化活性且无细胞毒性作用，证明它们在积极影响皮肤愈合方面具有广阔的潜力。
