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优化生长中的鹿茸的蛋白质产量。

Optimizing protein yield from growing deer antlers.

作者信息

Alegría-Aravena Nicolás, López-Garrido María-Pilar, Quiroz-Troncoso Josefa, González-Martos Raquel, Sánchez-Díez Marta, Gavira-O'Neill Clara, García-Díaz Andrés J, Landete-Castillejos Tomás, Ramírez-Castillejo Carmen, Chonco Louis, Sánchez-Sánchez Francisco

机构信息

Instituto de Desarrollo Regional (IDR), Instituto de Recursos Cinegéticos (IREC) and Escuela Técnica Superior de Ingeniéros Agrónomos y Montes y Biotecnología (ETSIAMB), University of Castilla-La Mancha (UCLM), Albacete, Spain.

Asociación Española Contra el Cáncer (AECC)-Fundación Científica AECC, Albacete, Spain.

出版信息

Front Bioeng Biotechnol. 2025 Jun 27;13:1612239. doi: 10.3389/fbioe.2025.1612239. eCollection 2025.

DOI:10.3389/fbioe.2025.1612239
PMID:40657160
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12245863/
Abstract

Growing antlers in deer contain bioactive compounds, most of which are proteins and peptides with effects on health, such as anticancer and regenerative properties. However, efficient extraction of these biomolecules while preserving their integrity remains a challenge. This study aimed to optimize the extraction of proteins from growing antlers through liquid removal methods, solvent selection, ratios, temperature, and extraction time. Lyophilization was identified as the optimal method for preserving protein integrity, particularly in biologically active regions. Among the tested solvents, water emerged as the most effective for protein extraction, achieving optimal results at a 1:10 w/v ratio with 1 hour of magnetic stirring at room temperature, although remains to be tested the anticancer effect of solvents different to water. Insulin-like growth factor 1 (IGF-1) was quantified as a key indicator of extraction efficiency, demonstrating that the optimized protocol effectively preserves this kind of bioactive protein. This methodology provides a robust framework for the extraction of proteins from growing antlers, paving the way for future applications in biomedical research.

摘要

鹿生长过程中的鹿角含有生物活性化合物，其中大部分是对健康有影响的蛋白质和肽，如具有抗癌和再生特性。然而，在保持这些生物分子完整性的同时高效提取它们仍然是一个挑战。本研究旨在通过液体去除方法、溶剂选择、比例、温度和提取时间来优化从生长鹿角中提取蛋白质的过程。冻干被确定为保持蛋白质完整性的最佳方法，特别是在生物活性区域。在所测试的溶剂中，水对蛋白质提取最为有效，在室温下以1:10 w/v的比例进行1小时磁力搅拌可获得最佳结果，不过与水不同的溶剂的抗癌效果仍有待测试。胰岛素样生长因子1（IGF-1）被定量为提取效率的关键指标，表明优化后的方案有效地保留了这种生物活性蛋白质。该方法为从生长鹿角中提取蛋白质提供了一个强大的框架，为未来在生物医学研究中的应用铺平了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/1bc48cf8001c/fbioe-13-1612239-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/f16119673aea/fbioe-13-1612239-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/aef677181b30/fbioe-13-1612239-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/15fcfc7003f1/fbioe-13-1612239-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/3dc0747a9a8a/fbioe-13-1612239-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/37fc2e669c32/fbioe-13-1612239-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/1bc48cf8001c/fbioe-13-1612239-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/f16119673aea/fbioe-13-1612239-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/8ff83b32507c/fbioe-13-1612239-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/1b4e6ed3b5c0/fbioe-13-1612239-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/aef677181b30/fbioe-13-1612239-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/15fcfc7003f1/fbioe-13-1612239-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/3dc0747a9a8a/fbioe-13-1612239-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/37fc2e669c32/fbioe-13-1612239-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e1/12245863/1bc48cf8001c/fbioe-13-1612239-g008.jpg

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本文引用的文献

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The signaling landscape of insulin-like growth factor 1.胰岛素样生长因子1的信号转导格局
J Biol Chem. 2025 Jan;301(1):108047. doi: 10.1016/j.jbc.2024.108047. Epub 2024 Dec 3.
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Effectiveness of Lyoprotectants in Protein Stabilization During Lyophilization.冻干过程中冻干保护剂对蛋白质的稳定作用
Pharmaceutics. 2024 Oct 21;16(10):1346. doi: 10.3390/pharmaceutics16101346.
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Enhancing organoid culture: harnessing the potential of decellularized extracellular matrix hydrogels for mimicking microenvironments.增强类器官培养：利用脱细胞细胞外基质水凝胶模拟微环境的潜力。
J Biomed Sci. 2024 Sep 27;31(1):96. doi: 10.1186/s12929-024-01086-7.
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Repair of Mechanical Cartilage Damage Using Exosomes Derived from Deer Antler Stem Cells.鹿茸干细胞来源的外泌体修复机械性软骨损伤。
Front Biosci (Landmark Ed). 2024 Aug 23;29(8):309. doi: 10.31083/j.fbl2908309.
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Sika Deer Velvet Antler Peptide Exerts Neuroprotective Effect in a Parkinson's Disease Model via Regulating Oxidative Damage and Gut Microbiota.梅花鹿鹿茸肽通过调节氧化损伤和肠道微生物群在帕金森病模型中发挥神经保护作用。
Pharmaceuticals (Basel). 2024 Jul 22;17(7):972. doi: 10.3390/ph17070972.
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Pharmaceutics. 2024 Apr 30;16(5):610. doi: 10.3390/pharmaceutics16050610.
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Antler stem cell-derived exosomes promote regenerative wound healing via fibroblast-to-myofibroblast transition inhibition.鹿角干细胞衍生的外泌体通过抑制成纤维细胞向肌成纤维细胞的转变促进再生性伤口愈合。
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