Beijing International Science and Technology Cooperation Base for Antiviral Drugs, Beijing Key Laboratory of Environmental and Viral Oncology, College of Chemistry and Life Science, Beijing University of Technology, Beijing, 100124, China.
School of Clinical Medicine, Beijing Tsinghua Changgung Hospital, Hepato-Pancreato-Biliary Center, Tsinghua University, Beijing, 102218, China.
J Biomed Sci. 2024 Sep 27;31(1):96. doi: 10.1186/s12929-024-01086-7.
Over the past decade, organoids have emerged as a prevalent and promising research tool, mirroring the physiological architecture of the human body. However, as the field advances, the traditional use of animal or tumor-derived extracellular matrix (ECM) as scaffolds has become increasingly inadequate. This shift has led to a focus on developing synthetic scaffolds, particularly hydrogels, that more accurately mimic three-dimensional (3D) tissue structures and dynamics in vitro. The ECM-cell interaction is crucial for organoid growth, necessitating hydrogels that meet organoid-specific requirements through modifiable physical and compositional properties. Advanced composite hydrogels have been engineered to more effectively replicate in vivo conditions, offering a more accurate representation of human organs compared to traditional matrices. This review explores the evolution and current uses of decellularized ECM scaffolds, emphasizing the application of decellularized ECM hydrogels in organoid culture. It also explores the fabrication of composite hydrogels and the prospects for their future use in organoid systems.
在过去的十年中，类器官已成为一种流行且有前途的研究工具，能够模拟人体的生理结构。然而，随着该领域的发展，传统上使用动物或肿瘤衍生的细胞外基质 (ECM) 作为支架已变得越来越不合适。这种转变促使人们专注于开发合成支架，特别是水凝胶，以更准确地在体外模拟三维 (3D) 组织结构和动力学。ECM-细胞相互作用对于类器官的生长至关重要，这需要通过可修改的物理和组成特性来满足类器官特定要求的水凝胶。先进的复合水凝胶已被设计用来更有效地复制体内条件，与传统基质相比，为人类器官提供了更准确的代表。本综述探讨了脱细胞 ECM 支架的演变和当前用途，强调了脱细胞 ECM 水凝胶在类器官培养中的应用。它还探讨了复合水凝胶的制造以及它们在类器官系统中的未来应用前景。
