Han Yuling, Yuan Guoyin, Yang Xiaolong, Fang Longfa, Liang Yu, Zhou Baoyuan, Wei Zhiyuan
Tropical Crop Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
National Key Laboratory for Tropical Crop Breeding, Sanya, China.
Front Microbiol. 2025 Jun 27;16:1615694. doi: 10.3389/fmicb.2025.1615694. eCollection 2025.
Arbuscular mycorrhizal fungi (AMF) substantially enhance soil fertility and are widely recognized as one of the most extensively researched biological inoculants. However, the effects of AMF on soil nutrient availability in mango orchards, along with the physiological processes regulating this availability under varying soil fertility conditions, remain poorly understood. To address this research gap, experiments were conducted with two soil types (soil from mango orchards co-cultivated with butterfly pea plants for 5 years) and a control (no butterfly pea plants) and two mycorrhizal inoculations (+AMF and -AMF). was used as the mycorrhizal inoculum. These experiments examined the physicochemical properties, microbial community composition, and metabolic profiles in mango orchard soils by integrating high-throughput sequencing with soil metabolomics. In nutrient-poor soils, the introduction of AMF enhanced the occurrence of specific bacterial species and elevated the richness and diversity of the soil bacterial community. This enhancement subsequently increased the activities of soil enzymes such as cellulase, -glucosidase, chitinase, and nitrate reductase in mycorrhizosphere soil, leading to improved soil pH, and increases in soil microbial biomass carbon (MBC), ammonium nitrogen (NH -N), nitrate nitrogen (NO -N), available potassium (K), and available phosphorus (P). Furthermore, alterations in soil properties and metabolites restructured the soil microbial community, with pH and MBC emerging as the key factors shaping bacterial distribution within mycorrhizosphere soil co-occurrence networks. In contrast, the effects of AMF on soil nutrient availability were weaker in high-fertility soils. We conclude that AMF enhance soil nutrient availability in mango orchards by regulating soil bacterial community structures, particularly in low-fertility soils.
丛枝菌根真菌（AMF）能显著提高土壤肥力，被广泛认为是研究最广泛的生物接种剂之一。然而，AMF对芒果园土壤养分有效性的影响，以及在不同土壤肥力条件下调节这种有效性的生理过程，仍知之甚少。为了填补这一研究空白，我们使用了两种土壤类型（与蝶豆植物共同种植5年的芒果园土壤）和一个对照（无蝶豆植物），以及两种菌根接种处理（+AMF和 -AMF）进行实验。 被用作菌根接种物。这些实验通过将高通量测序与土壤代谢组学相结合，研究了芒果园土壤的理化性质、微生物群落组成和代谢谱。在贫瘠土壤中，引入AMF增加了特定细菌种类的出现，并提高了土壤细菌群落的丰富度和多样性。这种增加随后提高了菌根际土壤中纤维素酶、β-葡萄糖苷酶、几丁质酶和硝酸还原酶等土壤酶的活性，导致土壤pH值改善，土壤微生物生物量碳（MBC）、铵态氮（NH₄⁺-N）、硝态氮（NO₃⁻-N）、速效钾（K）和速效磷（P）增加。此外，土壤性质和代谢物的变化重构了土壤微生物群落，pH值和MBC成为塑造菌根际土壤共现网络中细菌分布的关键因素。相比之下，在高肥力土壤中，AMF对土壤养分有效性的影响较弱。我们得出结论，AMF通过调节土壤细菌群落结构来提高芒果园土壤养分有效性, 尤其是在低肥力土壤中。
