Li Shasha, Jia Kefeng, Ao Siyu, Ma Huizhen, Zang Jiayan, Yan Haoyue, Xin Qi, Yan Yuxing, Ma Di, Huo Yuxuan, Yang Shuyu, Liu Ling, Li Shibo, Zhang Zili, Liu Pengfei, Wang Hao, Zhang Xiao-Dong
State Key Laboratory of Advanced Medical Materials and Devices, Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, China.
Central Hospital, Tianjin University, 83 Jintang Road, Hedong District, Tianjin 300170, China.
Nano Lett. 2025 Jul 23;25(29):11449-11459. doi: 10.1021/acs.nanolett.5c02725. Epub 2025 Jul 14.
Sepsis has a high global incidence and mortality, requiring early identification and assessment of organ damage. We present a NIR-II 3D visualization strategy using atomically precise fluorescent Au clusters to detect sepsis-induced multi-organ injury. These clusters emit strong fluorescence at 1044 nm with extended photostability (up to 300 min). Integrated with light-sheet microscopy, the method achieves rapid 3D imaging with a 2 μm isotropic resolution and 500 μm tissue penetration. It reveals significant pathological changes in two sepsis models, including intestinal villi shortening (to 32.7% and 43.8%) and renal tubule swelling (1.9× and 2.1×). Blood vessel integrity in pulmonary bronchioles was reduced to 37.1% and 45.2%. This approach enables detailed visualization of microstructural lesions and disease progression, underscoring its potential for early diagnosis and monitoring of sepsis-induced organ injury.
脓毒症在全球范围内具有较高的发病率和死亡率，需要早期识别和评估器官损伤。我们提出了一种使用原子精确的荧光金簇来检测脓毒症诱导的多器官损伤的近红外二区三维可视化策略。这些簇在1044nm处发射强烈荧光，具有延长的光稳定性（长达300分钟）。与光片显微镜相结合，该方法以2μm的各向同性分辨率和500μm的组织穿透深度实现快速三维成像。它揭示了两种脓毒症模型中的显著病理变化，包括肠绒毛缩短（至32.7%和43.8%）和肾小管肿胀（1.9倍和2.1倍）。肺细支气管中的血管完整性降低至37.1%和45.2%。这种方法能够详细可视化微观结构病变和疾病进展，突出了其在早期诊断和监测脓毒症诱导的器官损伤方面的潜力。
