Umetsu K, Ikeda N, Kashimura S, Suzuki T
Hum Genet. 1985;71(3):223-4. doi: 10.1007/BF00284578.
The genetic types of orosomucoid (ORM) were analyzed by isoelectric focusing (IEF) on polyacrylamide gels and subsequent print lectinofixation with a lectin from the beetle, allo A. In this paper, the newly devised print lectinofixation for ORM typing is described. This technique is faster, easier to perform, and has been found to be a useful tool in population genetics and forensic medicine. The results of typing for two alleles, ORM 1 and ORM 2 are described for a population of Northern Japan (n = 500).
通过在聚丙烯酰胺凝胶上进行等电聚焦(IEF)以及随后用来自甲虫异源A的凝集素进行印迹凝集素固定,分析了类粘蛋白(ORM)的遗传类型。本文描述了新设计的用于ORM分型的印迹凝集素固定方法。该技术速度更快、操作更简便,并且已被发现是群体遗传学和法医学中的一种有用工具。描述了日本北部一个群体(n = 500)中两个等位基因ORM 1和ORM 2的分型结果。