Luo X, Zhang B F, Mu Y J, Li Y F, Ma H X, Wang H F, Ye Y, Huang X Y, Guo W S
Infectious Disease Control and Prevention Institute, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China Henan Key Laboratory of Infectious Disease Microbiology, Zhengzhou 450016, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2025 Jul 6;59(7):989-996. doi: 10.3760/cma.j.cn112150-20240929-00783.
To investigate the pathogenetic characteristics of clinical isolates of in Henan Province from 2020 to 2023. Eight clinical isolates of in Henan Province from 2020 to 2023 were identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and real-time fluorescence polymerase chain reaction (PCR). Serotype and virulence genes were detected by the serum agglutination test and PCR, and antibiotic susceptibility was evaluated using the microbroth dilution method. Multilocus sequence typing (MLST), minimum core genome (MCG), identification of antibiotic resistance genes, and core genome single nucleotide polymorphism (cgSNP) analysis were conducted using whole genome sequencing. The results showed that eight strains isolated from humans were mainly serotype 2 (75.0%), while the rest were serotype 14 (25.0%). ST353 (62.5%) was the predominant genotype, followed by ST1 (25.0%) and ST7 (12.5%). All isolates belonged to the MCG1 group. The virulence genotypes of these isolates were primarily (NA2)/+/+/+(75.0%), while the remaining were (EU)/+/+/+(25.0%). These isolates carried tetracycline, macrolide, lincosamide and aminoglycoside resistance genes, and their resistance rates to tetracycline, erythromycin and clindamycin were 100.0%, 87.5% and 87.5%, respectively, and 62.5% strains were intermediate-resistant to penicillin. The cgSNP analysis indicated that these isolates were closer to the isolates from Guangdong, Zhejiang and Guangxi Provinces, with five ST353 strains and one ST7 strain belonging to Clade Ⅰ, and two ST1 strains belonging to Clade Ⅱ. The human isolates of in Henan Province are mainly ST353, harboring multiple virulence and antibiotic resistance genes.
为研究2020年至2023年河南省临床分离株的致病特征。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和实时荧光聚合酶链反应（PCR）对2020年至2023年河南省的8株临床分离株进行鉴定。通过血清凝集试验和PCR检测血清型和毒力基因，并使用微量肉汤稀释法评估抗生素敏感性。使用全基因组测序进行多位点序列分型（MLST）、最小核心基因组（MCG）、抗生素抗性基因鉴定和核心基因组单核苷酸多态性（cgSNP）分析。结果显示，从人类分离出的8株菌株主要为血清型2（75.0%），其余为血清型14（25.0%）。ST353（62.5%）是主要基因型，其次是ST1（25.0%）和ST7（12.5%）。所有分离株均属于MCG1组。这些分离株的毒力基因型主要为（NA2）/+/+/+（75.0%），其余为（EU）/+/+/+（25.0%）。这些分离株携带四环素、大环内酯、林可酰胺和氨基糖苷抗性基因，它们对四环素、红霉素和克林霉素的耐药率分别为100.0%、87.5%和87.5%，62.5%的菌株对青霉素呈中介耐药。cgSNP分析表明，这些分离株与来自广东、浙江和广西的分离株更接近，5株ST353菌株和1株ST7菌株属于进化枝Ⅰ，2株ST1菌株属于进化枝Ⅱ。河南省人类分离的菌株主要为ST353，携带多种毒力和抗生素抗性基因。
