Annefeld M
Int J Tissue React. 1985;7(4):273-89.
By means of a new ultrastructural test system, which is based on standardized morphometry and statistical evaluation, we are able for the first time to quantify changes in the cell metabolism of chondrocytes in the articular cartilage. To compare anti-inflammatory substances of different structure, different absorption characteristics and pharmacokinetics as regards their effect on rat cartilage, we used an equieffective dosage of the different anti-inflammatory drugs. Dexamethasone as steroidal and indomethacin and phenylbutazone as classical non-steroidal anti-inflammatory drugs (NSAIDs) were administered in an ED50 dosage referred to provoked arthritis over 12 weeks, using the same mode of administration. The standardized results of untreated rats weighing 300 and 450 g were used as the controls. Dexamethasone brings about massive degenerative changes in the ultrastructure of the vital chondrocyte. Under indomethacin and phenylbutazone the metabolic activity of the chondrocyte is inhibited to a much lesser extent. The damage to the chondrocyte after treatment with dexamethasone, indomethacin and phenylbutazone cannot be regarded as minimal but in some cases is tolerable as regards the benefit/risk ratio in the treatment of rheumatoid diseases.
借助一种基于标准化形态测量和统计评估的新型超微结构测试系统，我们首次能够量化关节软骨中软骨细胞的细胞代谢变化。为了比较不同结构、不同吸收特性和药代动力学的抗炎物质对大鼠软骨的影响，我们使用了不同抗炎药物的等效剂量。地塞米松作为甾体抗炎药，吲哚美辛和保泰松作为经典的非甾体抗炎药（NSAIDs），以针对诱发关节炎的ED50剂量，采用相同给药方式，在12周内给药。将体重300克和450克的未治疗大鼠的标准化结果用作对照。地塞米松会导致活软骨细胞超微结构发生大量退行性变化。在吲哚美辛和保泰松作用下，软骨细胞的代谢活性受到的抑制程度要小得多。用地塞米松、吲哚美辛和保泰松治疗后对软骨细胞造成的损伤不能被视为最小，但在某些情况下，就类风湿疾病治疗的获益/风险比而言是可以接受的。
