Al-Oebady Mouna Akee Hamed
Biology Department, College of Science, Al-Muthanna University, Samawah, Iraq.
Curr Med Mycol. 2024 Dec 31;10. doi: 10.22034/cmm.2024.345248.1586. eCollection 2024.
Various species of microorganisms interact in a variety of ecological niches and can lead to infection. A biofilm of one or more species may form during the infectious process. Otomycosis can be brought on by etiologic agents, such as and . This study aimed to survey the antagonistic relationship between the gene expression biofilms of and in the context of otomycosis-related biofilm formation.
This study examined single-species biofilms of and , as well as mixed-species biofilms of -, over 24 and 48 h. Expression of biofilm-related genes (, , , , , and ) was analyzed using real-time polymerase chain reaction (PCR). Impact of on the gene expression of was evaluated and compared to the gene expression of alone, which served as the control.
Biofilm formation assays showed that biofilm formation was significantly inhibited when co-cultured with , with optical density values dropping from 0.56 (alone) to 0.15 at 24 h and 0.05 at 48 h. Real-time PCR analysis revealed that the expression of biofilm-related genes, namely , , , , , and , increased significantly in single-species biofilms, reaching 2.5, 3, 1.5, 3.5, 2, and 1.7, respectively, at 24 h and 3.5, 4, 2, 4.2, 3, and 2, respectively at 48 h. However, in co-culture with , their gene expression was markedly reduced to 0.8, 0.5, 0.4, 0.9, 0.6, 0.5, respectively, at 24 h and 0.5, 1, 0.2, 0.8, 0.6. , and 0.3, respectively, at 48 h, demonstrating a strong inhibitory effect of on biofilm formation and gene expression.
This study described the antagonistic relationship between and on the gene expression biofilm that causes otomycosis, as well as the antibiosis relationship between the two during in vitro biofilm formation. These findings provide new insights into the complex interactions between these microorganisms during infection and may have implications for understanding and managing otomycosis.
多种微生物在各种生态位中相互作用，可导致感染。在感染过程中可能形成由一种或多种物种组成的生物膜。耳霉菌病可由病因病原体引起，如……和……。本研究旨在调查在与耳霉菌病相关的生物膜形成背景下，……和……的基因表达生物膜之间的拮抗关系。
本研究检测了……和……的单物种生物膜，以及…… - ……的混合物种生物膜，培养时间为24小时和48小时。使用实时聚合酶链反应（PCR）分析……生物膜相关基因（……、……、……、……、……和……）的表达。评估……对……基因表达的影响，并与单独的……基因表达进行比较，单独的……作为对照。
生物膜形成试验表明，与……共培养时，……的生物膜形成受到显著抑制，光密度值在24小时时从0.56（单独培养）降至0.15，在48小时时降至0.05。实时PCR分析显示，……生物膜相关基因，即……、……、……、……、……和……，在单物种生物膜中显著增加，在24小时时分别达到2.5、3、1.5、3.5、2和1.7，在48小时时分别达到3.5、4、2、4.2、3和2。然而，在与……共培养时，它们的基因表达在24小时时显著降低至0.8、0.5、0.4、0.9、0.6、0.5，在48小时时分别降至0.5、1、0.2、0.8、0.6、0.3，表明……对……生物膜形成和基因表达具有强烈的抑制作用。
本研究描述了……和……在导致耳霉菌病的基因表达生物膜上的拮抗关系，以及两者在体外生物膜形成过程中的抗菌关系。这些发现为这些微生物在感染过程中的复杂相互作用提供了新的见解，可能对理解和管理耳霉菌病具有重要意义。
