Wu Peipei, Lin Yu, Dai Fang, Wang Huming, Wen Hantao, Xu Zihan, Sun Gengyun, Lyu Zhaojie
The First Affiliated Hospital of Anhui Medical University, Peking University Shenzhen Hospital, PKU-Shenzhen Clinical Institute of Anhui Medical University, Shenzhen, 518036, China.
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China.
Discov Oncol. 2025 Jul 15;16(1):1335. doi: 10.1007/s12672-025-03026-9.
Abnormal expression of zinc finger (ZNF) proteins is closely associated with tumor proliferation and metastasis. However, due to limited knowledge about ZNF83, we conducted a pan-cancer analysis to explore its shared and distinct roles across various human malignancies.
Standardized TCGA pan-cancer data, encompassing clinical details and ZNF83 expression levels, were obtained for in-depth analysis. We assessed the expression landscape of ZNF83, explored its prognostic significance, and examined its relationship with tumor heterogeneity and cellular stemness. Furthermore, immunohistochemical analysis was performed on lung and kidney cancer tissues to support the bioinformatic findings.
ZNF83 expression patterns vary markedly among tumor types, with transcriptomic analysis revealing upregulation in eight malignancies and significant downregulation in twenty-two, compared to adjacent non-cancerous tissues. Notably, ZNF83 levels exhibited robust associations with markers indicative of genomic instability and with indices of tumor stemness across multiple cancer types, suggesting a role in genome maintenance and cellular plasticity. Immunohistochemical staining further substantiated these findings, showing elevated ZNF83 protein levels in renal carcinoma samples, whereas reduced expression was observed in lung adenocarcinoma specimens. In renal cancer, higher ZNF83 expression correlated with poorer overall survival, reinforcing its prognostic value. Although the difference in lung adenocarcinoma was not statistically significant, the expression trend was consistent with transcriptomic observations from the TCGA database.
ZNF83 is differentially expressed across cancers and predicts poor prognosis, particularly in renal cell carcinoma, highlighting its utility as a prognostic biomarker.
锌指（ZNF）蛋白的异常表达与肿瘤增殖和转移密切相关。然而，由于对ZNF83的了解有限，我们进行了一项泛癌分析，以探讨其在各种人类恶性肿瘤中的共同和独特作用。
获取标准化的TCGA泛癌数据，包括临床细节和ZNF83表达水平，进行深入分析。我们评估了ZNF83的表达格局，探讨了其预后意义，并研究了其与肿瘤异质性和细胞干性的关系。此外，对肺癌和肾癌组织进行免疫组化分析，以支持生物信息学研究结果。
ZNF83的表达模式在不同肿瘤类型之间存在显著差异，转录组分析显示，与相邻非癌组织相比，在8种恶性肿瘤中上调，在22种恶性肿瘤中显著下调。值得注意的是，ZNF83水平与指示基因组不稳定的标志物以及多种癌症类型中的肿瘤干性指数密切相关，表明其在基因组维持和细胞可塑性中发挥作用。免疫组化染色进一步证实了这些发现，显示肾癌样本中ZNF83蛋白水平升高，而在肺腺癌标本中观察到表达降低。在肾癌中，较高的ZNF83表达与较差的总生存期相关，强化了其预后价值。虽然肺腺癌的差异无统计学意义，但表达趋势与TCGA数据库的转录组观察结果一致。
ZNF83在不同癌症中差异表达，并预测不良预后，尤其是在肾细胞癌中，突出了其作为预后生物标志物的效用。
