Chi Yulei, Ren Wuang, Jin Pengkang, Shi Xuan, Liu Lan
School of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an, Shaanxi Province 710054, China.
School of Architecture and Civil Engineering, Xi'an University of Science and Technology, Xi'an, Shaanxi Province 710054, China.
Bioresour Technol. 2025 Nov;436:132975. doi: 10.1016/j.biortech.2025.132975. Epub 2025 Jul 13.
Low-strength nitrogen usually limits the efficiency of partial nitrification and anaerobic ammonium oxidation (anammox). To address this challenge, an organic-induced regulation strategy based on microbial interaction characteristics was proposed. Short-range addition of acetate for bio-screening is essential for achieving this regulation. After bio-screening, enhanced partial nitrification and anammox performance were achieved within 47 days. In the start-up phase, denitrifiers outcompeted nitrite oxidizing bacteria (NOB) for nitrite utilization, thereby enhancing the inhibition of NOB. The relative abundance of anammox bacteria (AnAOB) was maintained at approximately 8.6 % due to an increase in extracellular proteins, which was conducive to material exchange and symbiosis between denitrifiers and AnAOB. Transcriptomic analysis revealed that bio-screening induced mixotrophic metabolism of AnAOB, characterized by up-regulating the expression of genes involved in organic carbon and energy metabolism, thereby improving the stability of the anammox process. This study provides valuable insights for enhancing anammox performance under low-strength nitrogen conditions.
低强度氮通常会限制部分硝化和厌氧氨氧化（anammox）的效率。为应对这一挑战，提出了一种基于微生物相互作用特性的有机诱导调控策略。为实现这种调控，短程添加乙酸盐进行生物筛选至关重要。经过生物筛选，在47天内实现了强化的部分硝化和厌氧氨氧化性能。在启动阶段，反硝化菌在利用亚硝酸盐方面胜过亚硝酸盐氧化细菌（NOB），从而增强了对NOB的抑制。由于细胞外蛋白质增加，厌氧氨氧化菌（AnAOB）的相对丰度维持在约8.6%，这有利于反硝化菌与AnAOB之间的物质交换和共生。转录组分析表明，生物筛选诱导了AnAOB的混合营养代谢，其特征是上调参与有机碳和能量代谢的基因表达，从而提高了厌氧氨氧化过程的稳定性。本研究为在低强度氮条件下提高厌氧氨氧化性能提供了有价值的见解。
