• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

黑种草籽油及维生素C和E对黄曲霉毒素M诱导的新生大鼠血液学和肝脏毒性的保护作用

Protective role of nigella sativa oil and vitamins C and E against aflatoxin M-induced hematology and hepatic toxicity in neonatal rats.

作者信息

Badmos Amina Omodolapo, Oni Eniola Oluyemisi, Aladesida Adeyinka Adedeji, Oluwafemi Flora

机构信息

Department of Microbiology, Federal University of Agriculture, Abeokuta, 110124, Nigeria.

Department of Pure and Applied Zoology, Federal University of Agriculture, Abeokuta, 110124, Nigeria.

出版信息

Sci Rep. 2025 Jul 15;15(1):25611. doi: 10.1038/s41598-025-10778-5.

DOI:10.1038/s41598-025-10778-5
PMID:40664978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12264124/
Abstract

This study explores the protective efficacy of Nigella sativa oil (NSO), Vitamin C, and E in countering the toxic effects of Aflatoxin M1 (AFM1) in neonatal Wistar rats. A total of 117 neonatal rats were randomized into 13 groups (A-M) and observed over 12 weeks. Group A served as the control, while the remaining groups were exposed to varying concentrations of AFM1, either alone or in combination with the supplements. Hematological parameters, including Packed Cell Volume (PCV), Red Blood Cells (RBC), Hemoglobin (HB), White Blood Cells (WBC), and liver enzyme activities, were measured using Microhematocrit Reader, Hemocytometer, Hemoglobinometer, and Spectrophotometer respectively. Histopathological assessments of liver tissues were conducted using staining techniques to assess cellular damage, inflammation, and fibrosis. The results demonstrated that simultaneous supplementation with NSO, Vitamin C, and E significantly improved PCV, HB, and RBC levels while reducing WBC counts and liver enzyme activities in comparison to AFM1-exposed groups. Notably, NSO effectively restored hematological parameters and facilitated liver tissue regeneration, even at higher concentrations of AFM1. In contrast, Vitamins C and E displayed protective effects predominantly at lower toxin levels. These findings suggest that NSO, in conjunction with Vitamin C and E, plays a pivotal role in mitigating the detrimental effects of AFM1 toxicity. NSO shows superior potential in reversing blood dyscrasia and liver damage. The study highlights the promising therapeutic utility of Nigella sativa oil as a natural remedy for alleviating aflatoxin-induced oxidative stress and organ damage, reinforcing its potential to safeguard public health against aflatoxin exposure.

摘要

本研究探讨了黑种草籽油（NSO）、维生素C和维生素E对新生Wistar大鼠黄曲霉毒素M1（AFM1）毒性的保护作用。总共117只新生大鼠被随机分为13组（A - M），并观察12周。A组作为对照组，其余各组单独或与补充剂联合暴露于不同浓度的AFM1。分别使用微量血细胞比容读数仪、血细胞计数器、血红蛋白仪和分光光度计测量包括血细胞比容（PCV）、红细胞（RBC）、血红蛋白（HB）、白细胞（WBC）在内的血液学参数以及肝酶活性。使用染色技术对肝组织进行组织病理学评估，以评估细胞损伤、炎症和纤维化。结果表明，与暴露于AFM1的组相比，同时补充NSO、维生素C和维生素E可显著提高PCV、HB和RBC水平，同时降低WBC计数和肝酶活性。值得注意的是，即使在较高浓度的AFM1情况下，NSO也能有效恢复血液学参数并促进肝组织再生。相比之下，维生素C和维生素E主要在较低毒素水平时显示出保护作用。这些发现表明，NSO与维生素C和维生素E联合使用在减轻AFM1毒性的有害影响方面起着关键作用。NSO在逆转血液恶病质和肝损伤方面显示出卓越的潜力。该研究突出了黑种草籽油作为一种天然药物减轻黄曲霉毒素诱导的氧化应激和器官损伤的有前景的治疗效用，增强了其保护公众健康免受黄曲霉毒素暴露影响的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/f9bc65ca7e6f/41598_2025_10778_Fig17_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/0c174f8801bc/41598_2025_10778_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/d08c352e19bb/41598_2025_10778_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/7fd143d72132/41598_2025_10778_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/25afdf44c6ba/41598_2025_10778_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/f8f2e56dc4df/41598_2025_10778_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/128f349a568c/41598_2025_10778_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/b8a02667c0c3/41598_2025_10778_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/e3a49d2c20af/41598_2025_10778_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/8de18ca36016/41598_2025_10778_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/efb6e71e659b/41598_2025_10778_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/4a72c453c0ec/41598_2025_10778_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/9ddf7726aa69/41598_2025_10778_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/e3df663121e7/41598_2025_10778_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/6a8353f19a14/41598_2025_10778_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/2db62cf60b79/41598_2025_10778_Fig15_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/a8f83dad7bbe/41598_2025_10778_Fig16_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/f9bc65ca7e6f/41598_2025_10778_Fig17_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/0c174f8801bc/41598_2025_10778_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/d08c352e19bb/41598_2025_10778_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/7fd143d72132/41598_2025_10778_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/25afdf44c6ba/41598_2025_10778_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/f8f2e56dc4df/41598_2025_10778_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/128f349a568c/41598_2025_10778_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/b8a02667c0c3/41598_2025_10778_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/e3a49d2c20af/41598_2025_10778_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/8de18ca36016/41598_2025_10778_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/efb6e71e659b/41598_2025_10778_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/4a72c453c0ec/41598_2025_10778_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/9ddf7726aa69/41598_2025_10778_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/e3df663121e7/41598_2025_10778_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/6a8353f19a14/41598_2025_10778_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/2db62cf60b79/41598_2025_10778_Fig15_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/a8f83dad7bbe/41598_2025_10778_Fig16_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d5e/12264124/f9bc65ca7e6f/41598_2025_10778_Fig17_HTML.jpg

相似文献

1
Protective role of nigella sativa oil and vitamins C and E against aflatoxin M-induced hematology and hepatic toxicity in neonatal rats.黑种草籽油及维生素C和E对黄曲霉毒素M诱导的新生大鼠血液学和肝脏毒性的保护作用
Sci Rep. 2025 Jul 15;15(1):25611. doi: 10.1038/s41598-025-10778-5.
2
Neuroprotective role of Nigella Sativa seed oil in mitigating bisphenol a-induced neurodegeneration.黑种草籽油在减轻双酚A诱导的神经退行性变中的神经保护作用。
Sci Rep. 2025 Jul 2;15(1):22477. doi: 10.1038/s41598-024-80654-1.
3
Effects of on disease activity, T lymphocytes and inflammatory cytokine profiles in pediatric systemic lupus erythematosus: A randomized controlled trial.[具体物质]对儿童系统性红斑狼疮疾病活动、T淋巴细胞和炎性细胞因子谱的影响：一项随机对照试验
Narra J. 2024 Dec;4(3):e1063. doi: 10.52225/narra.v4i3.1063. Epub 2024 Nov 11.
4
Comparison of the Effectiveness of Nigella Sativa and Vitamin E in Preventing Intra-abdominal Adhesions.黑种草与维生素E预防腹腔内粘连的效果比较
Vet Med Sci. 2025 Jul;11(4):e70446. doi: 10.1002/vms3.70446.
5
Protective effect of hesperidin, ascorbic acid and their combination on oxidative stress, dyslipidemia, and histological changes in antitubercular drug-induced hepatotoxicity in rats.橙皮苷、抗坏血酸及其组合对大鼠抗结核药物性肝毒性中氧化应激、血脂异常及组织学变化的保护作用。
Indian J Pharmacol. 2025 Jan 1;57(1):4-11. doi: 10.4103/ijp.ijp_116_24. Epub 2025 May 6.
6
The effects of rose oil on liver damage in an experimental model of obstructive jaundice.玫瑰油对阻塞性黄疸实验模型中肝损伤的影响。
Ulus Travma Acil Cerrahi Derg. 2025 Jun;31(6):495-504. doi: 10.14744/tjtes.2025.96613.
7
Study on the modulation of kidney and liver function of rats with diabetic nephropathy by Huidouba through metabolomics.回豆巴通过代谢组学对糖尿病肾病大鼠肝肾功 能的调节作用研究
J Ethnopharmacol. 2025 Jun 11;351:120136. doi: 10.1016/j.jep.2025.120136.
8
Review, meta-analysis and carcinogenic risk assessment of aflatoxin M1 in different types of milks in Iran.伊朗不同类型牛奶中黄曲霉毒素 M1 的综述、荟萃分析和致癌风险评估。
Rev Environ Health. 2022 Jun 24;38(3):511-518. doi: 10.1515/reveh-2022-0050. Print 2023 Sep 26.
9
Manganese mitigates atrazine-induced oxidative stress and hepatorenal toxicity: insights from network pharmacology and in vivo experimentation.锰减轻莠去津诱导的氧化应激和肝肾毒性：来自网络药理学和体内实验的见解
BMC Pharmacol Toxicol. 2025 Jul 15;26(1):133. doi: 10.1186/s40360-025-00966-4.
10
Antioxidant vitamin and mineral supplements for preventing age-related macular degeneration.用于预防年龄相关性黄斑变性的抗氧化维生素和矿物质补充剂。
Cochrane Database Syst Rev. 2017 Jul 30;7(7):CD000253. doi: 10.1002/14651858.CD000253.pub4.

本文引用的文献

1
Contribution of Cyanotoxins to the Ecotoxicological Role of Lichens.蓝藻毒素在地衣生态毒理学作用中的贡献。
Toxins (Basel). 2021 Apr 29;13(5):321. doi: 10.3390/toxins13050321.
2
The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the relationship between aflatoxin B-DNA adducts in broilers.黑种草子和百里醌通过谷胱甘肽和谷胱甘肽-S-转移酶α-3对黄曲霉毒素相 2 解毒的影响，以及黄曲霉毒素 B-DNA 加合物在肉鸡中的关系。
Toxicon. 2021 Apr 15;193:86-92. doi: 10.1016/j.toxicon.2021.01.020. Epub 2021 Feb 11.
3
Impact of Mycotoxins on Animals' Oxidative Status.
霉菌毒素对动物氧化状态的影响。
Antioxidants (Basel). 2021 Feb 1;10(2):214. doi: 10.3390/antiox10020214.
4
Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0.报告动物研究：ARRIVE 指南 2.0 的解释和说明。
PLoS Biol. 2020 Jul 14;18(7):e3000411. doi: 10.1371/journal.pbio.3000411. eCollection 2020 Jul.
5
Aflatoxin B1 and M1: Biological Properties and Their Involvement in Cancer Development.黄曲霉毒素 B1 和 M1：生物学特性及其在癌症发展中的作用。
Toxins (Basel). 2018 May 24;10(6):214. doi: 10.3390/toxins10060214.
6
Aflatoxin M in human breast milk in Shahrekord, Iran and association with dietary factors.伊朗沙赫雷克德母乳中的黄曲霉毒素M及其与饮食因素的关联。
Food Addit Contam Part B Surveill. 2017 Jun;10(2):128-136. doi: 10.1080/19393210.2017.1282545. Epub 2017 Jan 29.
7
Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb(2+) using molecular beacon and DNAzyme based amplification strategy.基于分子信标和DNAzyme扩增策略的无酶无标记超灵敏比色法检测Pb(2+)
Biosens Bioelectron. 2016 Jun 15;80:187-193. doi: 10.1016/j.bios.2016.01.053. Epub 2016 Jan 22.
8
Survey of aflatoxin M₁ in cows' milk from free-grazing cows in Abeokuta, Nigeria.尼日利亚阿贝奥库塔自由放牧奶牛所产牛奶中黄曲霉毒素M₁的调查
Mycotoxin Res. 2014 Nov;30(4):207-11. doi: 10.1007/s12550-014-0204-4. Epub 2014 Jul 18.
9
A mini review on aflatoxin exposure in Malaysia: past, present and future.马来西亚黄曲霉毒素暴露情况的简要综述：过去、现在与未来
Front Microbiol. 2013 Nov 13;4:334. doi: 10.3389/fmicb.2013.00334.
10
Isolation of volatiles from Nigella sativa seeds using microwave-assisted extraction: effect of whole extracts on canine and murine CYP1A.利用微波辅助萃取法从黑种草籽中分离挥发物：全提取物对犬和小鼠细胞色素P450 1A的影响
Biomed Chromatogr. 2013 Jul;27(7):938-45. doi: 10.1002/bmc.2887. Epub 2013 Apr 30.