用于自组装和淀粉样蛋白纤维化研究的双功能纳米盘平台。
Bifunctional nanodisc platforms for studies on self-assembly and amyloid fibrillation.
作者信息
Sahoo Bikash R, Watanabe-Nakayama Takahiro, Suladze Saba, Anantharamaiah G M, Reif Bernd, Ramamoorthy Ayyalusamy
机构信息
Biophysics, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
WPI-Nano Life Science Institute, Kanazawa University, Kanazawa 9201192, Japan.
出版信息
Chem Commun (Camb). 2025 Aug 12;61(66):12349-12352. doi: 10.1039/d5cc00972c.
Abstract
Nanodiscs are emerging as valuable tools for studying lipid-protein interactions. In this study, we demonstrate the dual role of nanodiscs in modulating human amylin (hIAPP) fibrillation using NMR and HS-AFM. Peptide-based nanodiscs promote hIAPP fibrillation, while polymer-based nanodiscs inhibit aggregation. These findings broaden the applications of nanodiscs beyond structural studies.
摘要
纳米圆盘正成为研究脂质-蛋白质相互作用的重要工具。在本研究中，我们利用核磁共振（NMR）和高分辨率原子力显微镜（HS-AFM）证明了纳米圆盘在调节人胰岛淀粉样多肽（hIAPP）纤维化过程中的双重作用。基于肽的纳米圆盘促进hIAPP纤维化，而基于聚合物的纳米圆盘则抑制聚集。这些发现拓宽了纳米圆盘在结构研究之外的应用。
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