Mollabashi Melikasadat, Klopfer Alonza, Lunardon Thainá, Darzenta Nikolia, Davis Emily, Murray Matt, Sumner Scarlett M, Naskou Maria C
Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.
Front Vet Sci. 2025 Jul 1;12:1605649. doi: 10.3389/fvets.2025.1605649. eCollection 2025.
Platelet derived products have gained increasing attention as promising alternative biologicals for the treatment of canine wounds. Specifically, platelets play a crucial role during the inflammatory phase of wound healing due to the release of chemokines, proteins, cytokines, and growth factors. Additionally, platelets possess antimicrobial properties, which can be influenced by their manufacturing process, platelet and leukocyte concentration, activation method, and the presence of plasma and complement. The objective of this study was to assess how various preparation methods of platelet products affect their antimicrobial effect against bacteria commonly isolated from wounds.
In this study, blood was collected from eight purpose-bred dogs, and platelet-rich plasma was produced using two methods of centrifugation, one leukocyte-enriching and one leukocyte-reducing. Some samples were processed for plasma depletion and platelet lysate was subsequently generated through freeze-thaw cycles. Additionally, portions of platelet lysate samples underwent heat treatment for complement inactivation. All treatment groups were tested against four common bacteria found in canine skin wounds: , , and . The antimicrobial effect of various lysate formulations was evaluated using a bacteria-spiking (time-killing) assay.
Platelet lysate significantly reduced the number of and after 3 h compared to culture media. No significant differences were noted in the log reduction of bacteria between the centrifugation techniques. After depleting plasma, the log reduction of was significantly less than before plasma depletion, whereas the opposite was seen for after 3 h. Complement-depleted plasma led to a significantly lower log reduction for after 24 h compared to platelet lysate.
Therefore, the presence of plasma and complement proteins in platelet lysate appear to play a critical role in inhibiting the growth of certain bacterial strains, whereas the leukocyte concentration does not have a significant effect. Further research is needed to identify the ideal formulation and dose of canine platelet lysate as an antimicrobial and wound healing treatment.
血小板衍生产品作为治疗犬类伤口的有前景的替代生物制品，越来越受到关注。具体而言，血小板在伤口愈合的炎症阶段发挥着关键作用，因为它能释放趋化因子、蛋白质、细胞因子和生长因子。此外，血小板具有抗菌特性，这可能会受到其制造工艺、血小板和白细胞浓度、激活方法以及血浆和补体的存在的影响。本研究的目的是评估血小板产品的各种制备方法如何影响其对伤口常见分离细菌的抗菌效果。
在本研究中，从八只专门培育的犬只采集血液，使用两种离心方法制备富血小板血浆，一种是白细胞富集法，一种是白细胞减少法。对一些样本进行血浆去除处理，随后通过冻融循环产生血小板裂解物。此外，对部分血小板裂解物样本进行热处理以灭活补体。所有处理组均针对犬类皮肤伤口中发现的四种常见细菌进行测试： 、 、 和 。使用细菌加样（时间杀灭）试验评估各种裂解物制剂的抗菌效果。
与培养基相比，血小板裂解物在3小时后显著减少了 和 的数量。两种离心技术在细菌对数减少方面未观察到显著差异。去除血浆后， 的对数减少显著低于血浆去除前，而3小时后 的情况则相反。与血小板裂解物相比，补体去除血浆在24小时后导致 的对数减少显著更低。
因此，血小板裂解物中血浆和补体蛋白的存在似乎在抑制某些细菌菌株的生长中起关键作用，而白细胞浓度没有显著影响。需要进一步研究以确定作为抗菌和伤口愈合治疗的犬类血小板裂解物的理想制剂和剂量。
