草履虫细胞中的同步胞吐作用涉及到在具备胞吐能力的菌株中，一种65-kD磷蛋白非常快速（小于或等于1秒）的可逆去磷酸化。
Synchronous exocytosis in Paramecium cells involves very rapid (less than or equal to 1 s), reversible dephosphorylation of a 65-kD phosphoprotein in exocytosis-competent strains.
作者信息
Zieseniss E, Plattner H
出版信息
J Cell Biol. 1985 Dec;101(6):2028-35. doi: 10.1083/jcb.101.6.2028.
Abstract
Synchronous exocytosis in Paramecium cells involves the rapid (less than or equal to 1 s) dephosphorylation of a 65-kD phosphoprotein, which, after a lag phase of approximately 5 s, is reversed within approximately 20 s. Exocytosis inhibitors suppress this reaction; stimulatory and inhibitory effects are dose dependent. The dephosphorylation of the 65-kD phosphoprotein occurs only in exocytosis-competent strains, but not in mutant strains that cannot carry out membrane fusion, or that are devoid of secretory organelles or cannot transport them to the cell membrane. Since under all conditions analyzed the transient dephosphorylation of the 65-kD phosphoprotein strictly parallels the actual amount of exocytosed organelles, this process might be involved in exocytosis performance, perhaps in its initiation.
摘要
草履虫细胞中的同步胞吐作用涉及一种65-kD磷蛋白的快速（小于或等于1秒）去磷酸化，该去磷酸化在大约5秒的延迟期后，在大约20秒内逆转。胞吐作用抑制剂可抑制此反应；刺激和抑制作用呈剂量依赖性。65-kD磷蛋白的去磷酸化仅发生在具有胞吐能力的菌株中，而在不能进行膜融合、缺乏分泌细胞器或不能将其转运到细胞膜的突变菌株中则不会发生。由于在所有分析条件下，65-kD磷蛋白的瞬时去磷酸化与实际胞吐细胞器的数量严格平行，因此该过程可能参与胞吐作用，也许是其起始过程。
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Synchronous exocytosis in Paramecium cells involves very rapid (less than or equal to 1 s), reversible dephosphorylation of a 65-kD phosphoprotein in exocytosis-competent strains.草履虫细胞中的同步胞吐作用涉及到在具备胞吐能力的菌株中，一种65-kD磷蛋白非常快速（小于或等于1秒）的可逆去磷酸化。
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