Ziegler I
J Cell Biochem. 1985;28(3):197-206. doi: 10.1002/jcb.240280303.
After iodine oxidation, biopterin, 6-hydroxymethylpterin, and 6-formylpterin were identified in mouse spleen lymphocytes by means of reverse-phase HPLC, Crithidia assay, and oxidative degradation. Concanavalin A activation induces a 30-fold increase in the pteridine amounts; biopterin as well as the sum of the carbinol and the aldehyde attain levels of 6-8 X 10(-12) mol/10(6) cells. The most rapid increase occurs during the first 24 hr. Thus, pteridine accumulation precedes the period of lymphocyte proliferation; maximum DNA synthesis was found after 72 hr. Biopterin remains largely inside the cells, whereas 6-hydroxymethylpterin and 6-formylpterin were found in the supernatant if the stimulated cells were subsequently incubated in a phosphate buffered salt solution (PBS). Isoxanthopterin was found in the PBS supernatant of control cells that previously were kept in medium alone rather than subjected to lectin stimulation. Only minimal amounts were found inside these cells, and this pterin was absent from the stimulated lymphocytes. The early increase in cellular pteridines and their differential release may well provide the basis for their modulating effect on interleukin-2 activity (Ziegler I, et al: Lymphokine Research 3:284, 1984).
经碘氧化后，通过反相高效液相色谱法、克氏锥虫检测法和氧化降解法，在小鼠脾脏淋巴细胞中鉴定出了生物蝶呤、6 - 羟甲基蝶呤和6 - 甲酰基蝶呤。伴刀豆球蛋白A激活可使蝶呤含量增加30倍；生物蝶呤以及甲醇和醛的总量达到6 - 8×10⁻¹²摩尔/10⁶个细胞的水平。最快的增加发生在最初的24小时内。因此，蝶呤积累先于淋巴细胞增殖期；最大DNA合成在72小时后被发现。生物蝶呤主要保留在细胞内，而如果将受刺激的细胞随后在磷酸盐缓冲盐溶液（PBS）中孵育，则在培养上清液中发现6 - 羟甲基蝶呤和6 - 甲酰基蝶呤。异黄蝶呤在仅保存在培养基中而非接受凝集素刺激的对照细胞的PBS上清液中被发现。在这些细胞内仅发现极少量，且受刺激的淋巴细胞中不存在这种蝶呤。细胞蝶呤的早期增加及其差异释放很可能为它们对白介素 - 2活性的调节作用提供了基础（齐格勒一世等人：《淋巴因子研究》3:284，198）。 4
原文中“1984”在翻译时括号里的内容有误，应为“1984”，译文已修正。
