He Jiajun, Kong Lingyang, Wang Panpan, He Lianqing, Cao Huiyan, Qin Chen, Ren Weichao, Liu Xiubo, Ma Wei
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, 154000, China.
Mol Biol Rep. 2025 Jul 16;52(1):729. doi: 10.1007/s11033-025-10830-7.
The NAC gene family constitutes a distinct group of transcription factors exclusive to plants. They can regulate the growth and development of plants, respond to biotic and abiotic stresses, and participate in the regulation of secondary metabolites. Eleutherococcus senticosus is a plant with important medicinal value, and its active ingredients have shown significant effects in anti-fatigue, immune regulation, and neuroprotection. Analyzing the functions of E.senticosus NAC family genes is of great significance for revealing the molecular mechanism of synthesizing its medicinal ingredients and improving adaptability to adversity.
It was found that the EsNAC gene family contains a total of 118 members, which are distributed on 24 chromosomes. The 118 EsNACs are distributed in 16 subfamilies. The collinearity analysis reveals 112 instances of segmental duplication and 6 instances of tandem duplication within EsNAC.The analysis of cis-acting elements indicates that abscisic acid response elements rank as the most abundantly enriched, succeeded by methyl jasmonate response elements and anaerobic induction regulatory elements. Furthermore, MYB genes play a role in controlling the initiation of drought and the creation of flavonoids in EsNAC.Analysis of transcriptome data reveals that genes with significant expression are predominantly found within the two distinct subfamilies of ONAC022 and AtNAC3. The hypothesis suggests that the genetic roles of these two subfamilies might be involved in stress and augmenting the levels of secondary metabolites. Alterations in the expression of 10 EsNAC genes during salt stress were identified using qRT-PCR. The results show that salt stress particularly influences the expression of EsNAC genes.
This study provides important information for identifying the NAC gene family of E.senticosus and studying the effects of salt stress on the function of EsNAC genes.
NAC基因家族是植物特有的一类转录因子。它们能够调控植物的生长发育，响应生物和非生物胁迫，并参与次生代谢产物的调控。刺五加是一种具有重要药用价值的植物，其活性成分在抗疲劳、免疫调节和神经保护方面显示出显著效果。分析刺五加NAC家族基因的功能对于揭示其药用成分合成的分子机制以及提高其逆境适应性具有重要意义。
发现刺五加NAC基因家族共有118个成员，分布在24条染色体上。这118个刺五加NAC基因分布在16个亚家族中。共线性分析揭示了刺五加NAC基因内部有112个片段重复实例和6个串联重复实例。顺式作用元件分析表明，脱落酸响应元件富集程度最高，其次是茉莉酸甲酯响应元件和厌氧诱导调控元件。此外，MYB基因在刺五加NAC基因中对干旱起始和类黄酮生成的控制起作用。转录组数据分析表明，显著表达的基因主要存在于ONAC022和AtNAC3这两个不同的亚家族中。该假说认为，这两个亚家族的遗传作用可能与胁迫和次生代谢产物水平的提高有关。利用qRT-PCR鉴定了10个刺五加NAC基因在盐胁迫下的表达变化。结果表明，盐胁迫对刺五加NAC基因的表达有显著影响。
本研究为鉴定刺五加NAC基因家族以及研究盐胁迫对刺五加NAC基因功能的影响提供了重要信息。
