用于监测血浆中T-2毒素和HT-2毒素的具有药代动力学应用的气相色谱分析法。

Gas chromatographic assay with pharmacokinetic applications for monitoring T-2 and HT-2 toxins in plasma.

作者信息

Yagen B, Bialer M, Sintov A

出版信息

J Chromatogr. 1985 Sep 13;343(1):67-75. doi: 10.1016/s0378-4347(00)84569-7.

Abstract

A gas-liquid chromatographic (GLC) method for monitoring T-2 and HT-2 toxins in plasma was developed. The procedure involved extraction of the toxins with ethyl acetate, chromatography on a C18 reversed-phase column and derivatization with heptafluorobutyric anhydride (HFBA). The T-2 and HT-2 HFBA derivatives were chromatographed on OV-17 at various temperatures and measured with an electron-capture detector. Iso-T-2 toxin and iso-HT-2 toxin were used as internal standards. Recoveries averaged 95.1 +/- 8.6% for T-2 toxin and 102.1 +/- 5.2% for HT-2 toxin at levels ranging from 40 to 120 ng/ml. The limits of detection were 30 and 5 ng/ml of T-2 and HT-2 toxin, respectively. The range of the assay covers plasma concentrations at which toxicity becomes manifest. The pharmacokinetic application of this GLC method is illustrated by simultaneous monitoring of T-2 and HT-2 toxins levels in plasma obtained after intravenous administration of T-2 toxin to a dog.

摘要

建立了一种用于监测血浆中T-2和HT-2毒素的气液色谱（GLC）方法。该方法包括用乙酸乙酯萃取毒素，在C18反相柱上进行色谱分离，并用七氟丁酸酐（HFBA）进行衍生化。T-2和HT-2的HFBA衍生物在不同温度下于OV-17上进行色谱分离，并用电子捕获检测器进行测定。异T-2毒素和异HT-2毒素用作内标。在40至120 ng/ml的浓度范围内，T-2毒素的回收率平均为95.1±8.6%，HT-2毒素的回收率平均为102.1±5.2%。T-2和HT-2毒素的检测限分别为30和5 ng/ml。该检测方法的范围涵盖了毒性显现时的血浆浓度。通过对一只狗静脉注射T-2毒素后获得的血浆中T-2和HT-2毒素水平进行同步监测，说明了该GLC方法在药代动力学中的应用。