Swanson S P, Ramaswamy V, Beasley V R, Buck W B, Burmeister H H
J Assoc Off Anal Chem. 1983 Jul;66(4):909-12.
A gas-liquid chromatographic method for the determination of T-2 toxin in plasma is described. The toxin is extracted with benzene, washed with aqueous sodium hydroxide, and chromatographed on a small Florisil column; the heptafluorobutyryl derivative is prepared by reaction with heptafluorobutyrylimidazole. The T-2 HFB derivative is chromatographed on OV-1 at 230 degrees C and measured with an electron capture detector. Iso-T-2, an isomer of T-2 toxin, is added to samples as an internal standard before extraction. Recoveries averaged 98.0 +/- 5.5% at levels ranging from 50 to 1000 ng/mL. The limit of detection is 25 ng/mL.
描述了一种用于测定血浆中T-2毒素的气-液色谱法。毒素用苯萃取，用氢氧化钠水溶液洗涤，然后在小型弗罗里硅土柱上进行色谱分析；通过与七氟丁酰咪唑反应制备七氟丁酰衍生物。T-2 HFB衍生物在230℃下于OV-1上进行色谱分析，并用电子捕获检测器进行测定。在萃取前，将T-2毒素的异构体异T-2作为内标加入样品中。在50至1000 ng/mL的浓度范围内，回收率平均为98.0±5.5%。检测限为25 ng/mL。
