Minagawa K, Kasuya Y, Baba S, Knapp G, Skelly J P
J Chromatogr. 1985 Oct 11;343(2):231-7. doi: 10.1016/s0378-4347(00)84593-4.
A gas chromatographic-electron-impact mass spectrometric method for the determination of dexamethasone in biological fluids has been developed. Quantitation by isotopedilution mass spectrometry was carried out by selected-ion monitoring on the molecular ions of the tetra(trimethylsilyl) derivative of dexamethasone and of dexamethasone M+9 (m/z 680 and 689, respectively). The sensitivity, specificity, precision and accuracy of the method were demonstrated to be satisfactory for application to pharmacokinetic and bioavailability studies of dexamethasone after administration of a therapeutic dose.
已开发出一种用于测定生物流体中地塞米松的气相色谱-电子轰击质谱法。通过对氘代地塞米松和地塞米松M+9（分别为m/z 680和689）的四（三甲基硅基）衍生物的分子离子进行选择离子监测，采用同位素稀释质谱法进行定量。该方法的灵敏度、特异性、精密度和准确度经证明对于应用于治疗剂量给药后地塞米松的药代动力学和生物利用度研究而言是令人满意的。
