Tsuei S E, Ashley J J, Moore R G, McBride W G
J Chromatogr. 1978 Mar 1;145(2):213-20. doi: 10.1016/s0378-4347(00)81341-9.
A sensitive and specific method for the quantitation of dexamethasone in plasma and urine is described. The specificity of the method is obtained using adsoprtion chromatography on a high-performance liquid chromatograph. The dexamethasone is detected with a variable-wavelength UV detector. An internal standard technique is used for quantitation of dexamethasone with a minimum sensitivity of 15 ng. Preliminary results of the application of the method to pharmacokinetic studies of dexamethasone in humans are reported.
本文描述了一种灵敏且特异的血浆和尿液中地塞米松定量方法。该方法的特异性通过在高效液相色谱仪上进行吸附色谱获得。地塞米松用可变波长紫外检测器进行检测。采用内标技术定量地塞米松，最低灵敏度为15纳克。报告了该方法应用于地塞米松人体药代动力学研究的初步结果。
