Bouvier C A, Gabbiani G, Ryan G B, Badonnel M C, Majno G, Lüscher E F
Thromb Haemost. 1977 Apr 30;37(2):321-8.
Normal platelets incubated with anti-actin autoantibodies (AAA) (from the serum of patients with chronic aggressive hepatitis) do not show binding of these antibodies as seen by indirect immunofluorescence. AAA serum does not inhibit thrombin-induced clot retraction, despite the binding of the antibodies to platelets in the clot. Similarly, AAA serum does not affect "reversible" or "irreversible" aggregation (induced by ADP, collagen or epinephrine), despite the binding of the antibodies to platelet actin under such circumstances. AAA also bind to platelets when aggregation is inhibited by EDTA. The incubation of "reversibly" aggregated platelet with AAA results in a small but definite binding of AAA to platelets. These findings suggest that during "irreversible" and/or "reversible" aggregation, changes take place at the surface of platelets which expose the antigen at the surface of the cell.
用抗肌动蛋白自身抗体（AAA）（来自慢性侵袭性肝炎患者的血清）孵育正常血小板时，通过间接免疫荧光观察，未显示出这些抗体的结合。尽管抗体与凝块中的血小板结合，但AAA血清并不抑制凝血酶诱导的凝块回缩。同样，尽管在这种情况下抗体与血小板肌动蛋白结合，但AAA血清并不影响（由ADP、胶原蛋白或肾上腺素诱导的）“可逆”或“不可逆”聚集。当聚集被EDTA抑制时，AAA也会与血小板结合。用AAA孵育“可逆”聚集的血小板会导致AAA与血小板有少量但确定的结合。这些发现表明，在“不可逆”和/或“可逆”聚集过程中，血小板表面会发生变化，从而使细胞表面的抗原暴露出来。
