He Zhengxiao, Fahey Alan G, Liu Ranran, Wen Jie, Zhao Guiping
State Key Laboratory of Animal Biotech Breeding; State Key Laboratory of Animal Nutrition and Feeding; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
Poult Sci. 2025 Jul 3;104(10):105521. doi: 10.1016/j.psj.2025.105521.
Short-chain fatty acids (SCFAs) are essential metabolites which play an important role in biological functions such as energy homeostasis and immune function. However, duodenum, liver and serum have different biological functions, and SCFAs were also detected in these sites. This study aims to compare the SCFA composition of the liver, serum, and duodenum and estimate the correlation between SCFAs and residual feed intake (RFI) in broilers.
Broiler chickens from a commercial breeding paternal line were used in this study. The liver, serum, duodenum and cecum SCFA concentrations were detected through the LC-MS method. Cecum microbe was sequenced through the V4 region and annotated by the SLIVA_123 database. The co-occurrence networks were based on the Spearman correlations, and the bi-direction two-sample Mendelian randomisation method (TMR) was used to investigate the causal effect between SCFAs and RFI.
Liver, duodenum, and serum content had similar SCFA compositions. Compared to the other three sites, the cecum contains a variety of microbiota, as the different SCFAs are produced by microbiota. The causal investigation between SCFA with RFI were first investigated in this study. The bidirectional Mendelian randomisation results illustrated that RFI could have a causal effect on four cecum SCFAs. In contrast, there is no causal effect of cecum SCFAs on RFI disagreed with many nutritional trials, and the contradiction needs further investigation.
短链脂肪酸（SCFAs）是重要的代谢产物，在能量平衡和免疫功能等生物学功能中发挥着重要作用。然而，十二指肠、肝脏和血清具有不同的生物学功能，并且在这些部位也检测到了短链脂肪酸。本研究旨在比较肝脏、血清和十二指肠中的短链脂肪酸组成，并评估短链脂肪酸与肉鸡剩余采食量（RFI）之间的相关性。
本研究使用了来自商业育种父系的肉鸡。通过液相色谱-质谱法检测肝脏、血清、十二指肠和盲肠中的短链脂肪酸浓度。通过V4区域对盲肠微生物进行测序，并由SLIVA_123数据库进行注释。共现网络基于斯皮尔曼相关性，采用双向双样本孟德尔随机化方法（TMR）研究短链脂肪酸与RFI之间的因果效应。
肝脏、十二指肠和血清中的短链脂肪酸组成相似。与其他三个部位相比，盲肠含有多种微生物群，因为不同的短链脂肪酸是由微生物群产生的。本研究首次对短链脂肪酸与RFI之间的因果关系进行了研究。双向孟德尔随机化结果表明，RFI可能对四种盲肠短链脂肪酸有因果效应。相比之下，盲肠短链脂肪酸对RFI没有因果效应，这与许多营养试验不一致，这种矛盾需要进一步研究。
