Teng S H, Andrews A G, Horacek I
J Clin Pathol. 1985 Nov;38(11):1304-8. doi: 10.1136/jcp.38.11.1304.
The relation between the choline containing surfactant phospholipids lecithin and sphingomyelin in amniotic fluid and fetal lung maturity is well established. An enzymatic method that had been automated and optimised for use on a centrifugal analyser was used to measure the total choline containing phospholipids in amniotic fluid. The total time taken for this assay was 10 minutes. The results obtained from 100 patient samples, using this procedure, compared favourably with the results obtained by the thin layer chromatography procedure used to determine the lecithin to sphingomyelin ratio (r = 0.93). A clinical study of 60 patients showed that this assay predicted prenatal respiratory distress syndrome as well as the lecithin to sphingomyelin ratios. The advantage of this assay over existing procedures is that it requires minimum preparation of the specimen and no extraction, is quick, and shows a high degree of precision.
羊水内含胆碱的表面活性磷脂卵磷脂和鞘磷脂与胎儿肺成熟度之间的关系已得到充分证实。一种已实现自动化并针对离心分析仪进行优化的酶法被用于测量羊水中总含胆碱磷脂。该检测的总用时为10分钟。采用此方法从100例患者样本中获得的结果，与通过用于测定卵磷脂与鞘磷脂比率的薄层色谱法获得的结果相比，效果良好（r = 0.93）。一项针对60例患者的临床研究表明，该检测对产前呼吸窘迫综合征的预测效果与卵磷脂与鞘磷脂比率相当。此检测相对于现有方法的优势在于，它所需的样本预处理最少且无需提取，速度快，并且具有高度的精确性。
