Artiss J D, McGowan M W, Strandbergh D R, Epstein E, Zak B
Clin Chem. 1984 Apr;30(4):534-7.
We describe a procedure for the enzymic, colorimetric determination of phosphatidylglycerol in amniotic fluid. After extraction into chloroform:methanol (2:1 by vol) and evaporation, the phospholipid-containing residue is redissolved in a non-ionic detergent, which thus provides an aqueous sample. The subsequent enzymic reaction sequence involves phospholipase-catalyzed hydrolysis of glycerol from its phospholipid. Subsequent enzyme-catalyzed reactions phosphorylate this glycerol and oxidize the resulting glycerol phosphate to produce hydrogen peroxide, which is reacted to produce an intense red chromogen in the peroxidase-catalyzed coupling of 4-aminoantipyrine and 2-hydroxy-3,5-dichlorobenzenesulfonate. When used in conjunction with previously reported enzymic techniques for determination of lecithin and sphingomyelin, this procedure may provide an accurate and precise "lung profile" for assessment of fetal lung maturity.
我们描述了一种用于羊水磷脂酰甘油酶促比色测定的方法。将羊水用氯仿：甲醇（体积比2:1）萃取并蒸发后，含磷脂的残余物重新溶解于非离子型去污剂中，从而得到水性样品。随后的酶促反应序列包括磷脂酶催化从磷脂中水解出甘油。随后的酶催化反应使该甘油磷酸化，并将生成的甘油磷酸氧化以产生过氧化氢，在过氧化物酶催化的4-氨基安替比林与2-羟基-3,5-二氯苯磺酸盐的偶联反应中，过氧化氢反应生成强烈的红色色原。当与先前报道的用于测定卵磷脂和鞘磷脂的酶促技术结合使用时，该方法可为评估胎儿肺成熟度提供准确且精确的“肺图谱”。
