Zhang Yuting, Zhang Ze, Zhang Hehua, Xu Xin, Shen Tong, An Xiaohui, Pan Hongzhi, Chang Dong
Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China; Department of Clinical Laboratory, Zhangye People's Hospital affiliated to Hexi University, Zhangye, 734000, China.
Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China.
Anal Chim Acta. 2025 Sep 22;1368:344298. doi: 10.1016/j.aca.2025.344298. Epub 2025 Jun 6.
Circulating tumor cells (CTCs), as a critical biomarker for early diagnosis and real-time monitoring of tumor progression, play a significant role in early cancer diagnosis, treatment monitoring, and prognosis evaluation. However, existing CTCs detection methods are limited by low purity, long processing time, high cost, and insufficient specificity, making them inadequate for clinical needs. This study developed an ultrasensitive and visually detectable CTCs sensing platform. The platform integrates nucleic acid aptamer-functionalized dopamine-coated magnetic nanoparticles as capture probes for the specific isolation and enrichment of CTCs, and MXenes@MnCoZDH@Au NPs as signal-amplifying nanoprobes with exceptional peroxidase-like activity. The colorimetric sensing mechanism relies on the linear increase in absorbance with CTCs concentration, enabling quantitative detection. Under optimized experimental conditions, the sensor demonstrated a broad detection range of 10 to 10 cells/mL and an ultra-low 2 cells/mL detection limit. Compared to traditional methods, this detection approach exhibited high specificity, sensitivity, and reproducibility, highlighting its potential for clinical translation. This innovative method provides a reliable and practical tool for liquid biopsy-based cancer diagnostics, offering significant advancements in precision medicine.
循环肿瘤细胞（CTCs）作为肿瘤早期诊断和实时监测的关键生物标志物，在癌症早期诊断、治疗监测和预后评估中发挥着重要作用。然而，现有的CTCs检测方法受到纯度低、处理时间长、成本高和特异性不足的限制，无法满足临床需求。本研究开发了一种超灵敏且可视觉检测的CTCs传感平台。该平台整合了核酸适配体功能化的多巴胺包覆磁性纳米颗粒作为捕获探针，用于CTCs的特异性分离和富集，以及MXenes@MnCoZDH@Au NPs作为具有优异类过氧化物酶活性的信号放大纳米探针。比色传感机制依赖于吸光度随CTCs浓度的线性增加，从而实现定量检测。在优化的实验条件下，该传感器展示了10至10⁵个细胞/毫升的宽检测范围和2个细胞/毫升的超低检测限。与传统方法相比，这种检测方法具有高特异性、灵敏度和重现性，突出了其临床转化的潜力。这种创新方法为基于液体活检的癌症诊断提供了一种可靠且实用的工具，在精准医学方面取得了重大进展。
