Jesumirhewe Christiana, Cabal-Rosel Adriana, Allerberger Franz, Springer Burkhard, Ruppitsch Werner
Department of Pharmaceutical Microbiology, College of Pharmacy, Igbinedion University, Okada, Edo state, Nigeria.
Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Vienna, Austria.
Access Microbiol. 2023 Jul 12;5(7). doi: 10.1099/acmi.0.000509.v4. eCollection 2023.
The emergence of antibiotic resistance in livestock, especially food-producing animals, is of major public health importance as a result of the possibility of these bacteria entering the food chain. In this study, the genetic characteristics of antibiotic-resistant and spp. isolates from humans and poultry in Edo state, Nigeria, were investigated. In April 2017, 45 spp. and 46 isolates were obtained from urine, clinical wounds, nasal and chicken faecal samples. Isolates were recovered and identified as previously described. Species identification was achieved by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and ribosomal multilocus sequence typing. Antimicrobial susceptibility testing was carried out using the Kirby-Bauer method for 12 antibiotics. A double disc synergy test was used to screen for extended-spectrum beta-lactamse (ESBL) production. Whole genome sequencing was performed for strain characterization of the isolates. Thirteen spp. isolates yielded positive results by the ESBL phenotypic test and harboured ESBL genes. Of the 46 isolates, 21 human and 13 poultry isolates were resistant to at least one of the tested antibiotics. Four human isolates harboured ESBL genes and revealed positive results when applying ESBL double disc synergy tests. ESBL genes in the spp. and isolates include and . Whole genome-based core gene multilocus sequence typing of the spp. and isolates revealed a close relatedness among the isolates. An integrated 'One Health' surveillance system is required to monitor transmission of antimicrobial resistance in Nigeria.
家畜尤其是食用动物中抗生素耐药性的出现具有重大公共卫生意义，因为这些细菌有可能进入食物链。在本研究中，对尼日利亚江户州人类和家禽中抗生素耐药性 和 属分离株的遗传特征进行了调查。2017年4月，从尿液、临床伤口、鼻腔和鸡粪便样本中获得了45株 属和46株 分离株。分离株的回收和鉴定如前所述。通过基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱和核糖体多位点序列分型进行物种鉴定。使用Kirby-Bauer方法对12种抗生素进行药敏试验。采用双纸片协同试验筛选超广谱β-内酰胺酶（ESBL）的产生。对分离株进行全基因组测序以进行菌株特征分析。13株 属分离株通过ESBL表型试验获得阳性结果并携带ESBL基因。在46株 分离株中，21株人类分离株和13株家禽分离株对至少一种受试抗生素耐药。4株人类 分离株携带ESBL基因，在应用ESBL双纸片协同试验时显示阳性结果。 属和 分离株中的ESBL基因包括 和 。基于全基因组的 属和 分离株核心基因多位点序列分型显示分离株之间密切相关。需要一个综合的“同一健康”监测系统来监测尼日利亚抗菌药物耐药性的传播。
