由……和……介导的环二鸟苷酸依赖性运动调节

c-di-GMP-Dependent Regulation of Motility by and .

作者信息

Hahn Jeanette, Celma Louisa, Elshereef Abdalla A, Samir Sherihan, Dubnau Eugenie, Selim Khaled A, Dubnau David

机构信息

Public Health Research Institute, Rutgers University, Newark, New Jersey, USA.

Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.

出版信息

bioRxiv. 2025 Jul 11:2025.07.11.664319. doi: 10.1101/2025.07.11.664319.

DOI:10.1101/2025.07.11.664319

PMID:40672238

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12265714/

Abstract

ComFB is encoded in the operon of , situated between the genes for ComFA and ComFC. The latter two proteins are essential for natural transformation, whereas ComFB is dispensable. We show here that ComFB binds specifically and with high affinity to the second messenger c-di-GMP and that ComFB acts as a c-di-GMP receptor to inhibit swarming and swimming motility, apparently by interfering with flagellar activity. We show further that in the absence of ComFC, swarming is completely abrogated by a mechanism that requires FB. These results reveal a new c-di-GMP regulatory system that controls motility independently of MotI.

摘要

ComFB 由位于 ComFA 和 ComFC 基因之间的操纵子编码。后两种蛋白质对自然转化至关重要，而 ComFB 则是可有可无的。我们在此表明，ComFB 与第二信使 c-di-GMP 特异性结合且亲和力高，并且 ComFB 作为 c-di-GMP 受体发挥作用，通过干扰鞭毛活性来抑制群体运动和游动运动。我们进一步表明，在没有 ComFC 的情况下，群体运动通过一种需要 FB 的机制被完全消除。这些结果揭示了一种新的 c-di-GMP 调节系统，该系统独立于 MotI 控制运动。