Feng Hexiang, Zhong Linjun, Yang Xinjian, Wu Haokun, Sun Qiankai, Wei Hao, He Yinbo, Zhou Linsen, Zhang Yusong, Li Mei, Sun Yu
West China School of Medicine, Sichuan University, Chengdu, China.
Department of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu, China.
Front Nutr. 2025 Jul 2;12:1613132. doi: 10.3389/fnut.2025.1613132. eCollection 2025.
This study investigated the effects of a compound preparation combining and probiotics (LB-Pro) on chemotherapy-induced cancer-related fatigue (CRF) in a murine model. The aim was to explore potential mechanisms related to the gut microbiota-metabolic axis.
A CRF model was established in C57BL/6NCr mice using 5-fluorouracil. Mice were divided into four groups receiving varying concentrations of LB-Pro. Over 14 days, interventions were administered, followed by treadmill exhaustion tests to assess fatigue levels. Body weight, serum biomarkers (TNF-, GSH-Px, NAD-MDH, SOD), and gut microbiota composition were analyzed to evaluate physiological and metabolic changes.
Administration of medium- and high-concentration LB-Pro significantly improved fatigue-related outcomes, including prolonged exhaustion times and enhanced antioxidant enzyme activities, compared to the control group. Low-concentration LB-Pro showed limited efficacy. Gut microbiota analysis revealed alterations in microbial composition, including enrichment of short-chain fatty acid-producing taxa, and metabolic pathways associated with energy metabolism and antioxidant defense were upregulated in probiotic-treated groups.
LB-Pro alleviated chemotherapy-induced CRF in mice, likely through modulation of gut microbiota and enhancement of mitochondrial energy metabolism and antioxidant systems. These findings highlight the potential of integrative approaches combining traditional Chinese medicine and probiotics for managing CRF, emphasizing the gut microbiota-metabolic axis as a key therapeutic target.
本研究在小鼠模型中调查了一种将[具体成分]与益生菌结合的复合制剂（LB-Pro）对化疗引起的癌症相关疲劳（CRF）的影响。目的是探索与肠道微生物群-代谢轴相关的潜在机制。
使用5-氟尿嘧啶在C57BL/6NCr小鼠中建立CRF模型。将小鼠分为四组，给予不同浓度的LB-Pro。在14天内进行干预，随后进行跑步机耐力测试以评估疲劳水平。分析体重、血清生物标志物（TNF-、GSH-Px、NAD-MDH、SOD）和肠道微生物群组成，以评估生理和代谢变化。
与对照组相比，中、高浓度LB-Pro给药显著改善了与疲劳相关的结果，包括延长耐力时间和增强抗氧化酶活性。低浓度LB-Pro显示出有限的疗效。肠道微生物群分析揭示了微生物组成的改变，包括产生短链脂肪酸的分类群的富集，并且在益生菌治疗组中与能量代谢和抗氧化防御相关的代谢途径上调。
LB-Pro减轻了小鼠化疗诱导的CRF，可能是通过调节肠道微生物群以及增强线粒体能量代谢和抗氧化系统。这些发现突出了将中药和益生菌相结合的综合方法在管理CRF方面的潜力，强调肠道微生物群-代谢轴作为关键治疗靶点。
