Sciorra L J, Lee M L, Wynnyckyj H
J Histochem Cytochem. 1985 Dec;33(12):1252-5. doi: 10.1177/33.12.4067277.
We describe a method for labeling chromosomal proteins with an amino-group-specific fluorescent reagent, fluorescamine. Chromosomes thus labeled appear either as uniformly fluorescent or as haloes in structure depending on the proteins remaining after treatment with acid-alcohol fixation. Using fluorescamine as a probe, we demonstrate that there is a substantial loss of labeled proteins during the chromosomal preparation and also during the trypsin treatment used in the banding of chromosomes.
我们描述了一种用氨基特异性荧光试剂荧光胺标记染色体蛋白质的方法。如此标记的染色体根据酸 - 乙醇固定处理后残留的蛋白质，呈现出均匀荧光或结构上的光晕。使用荧光胺作为探针，我们证明在染色体制备过程以及用于染色体显带的胰蛋白酶处理过程中，标记蛋白质有大量损失。
