Sjögren-Jansson E, Jeansson S
J Immunol Methods. 1985 Nov 28;84(1-2):359-64. doi: 10.1016/0022-1759(85)90442-9.
A simple procedure for the large-scale production of monoclonal antibodies has been developed. Hybridomas were grown in dialysis tubing containing medium with a low concentration of proteins. The dialysis tubing was inserted into a flask with medium containing 10% or 30% foetal calf serum. The flask was placed on a roller and medium was changed every other day. Monoclonal antibodies were harvested after about 10 days in culture. Immunoglobulin concentrations up to 5.4 mg/ml and cell yields of 85 X 10(6) cells/ml have been obtained. The low concentration of contaminating low molecular weight proteins in the supernatant from cells grown in dialysis tubing facilitated purification of the monoclonal antibodies.
已开发出一种用于大规模生产单克隆抗体的简单方法。杂交瘤细胞在含有低浓度蛋白质的培养基的透析袋中培养。将透析袋插入含有10%或30%胎牛血清的培养基的烧瓶中。将烧瓶置于滚轴上，每隔一天更换一次培养基。培养约10天后收获单克隆抗体。已获得高达5.4 mg/ml的免疫球蛋白浓度和85×10⁶个细胞/ml的细胞产量。在透析袋中培养的细胞的上清液中低分子量污染蛋白的浓度低，便于单克隆抗体的纯化。
