Fredman P, Mattsson L, Andersson K, Davidsson P, Ishizuka I, Jeansson S, Månsson J E, Svennerholm L
Department of Psychiatry and Neurochemistry, University of Gothenburg, Sweden.
Biochem J. 1988 Apr 1;251(1):17-22. doi: 10.1042/bj2510017.
An IgG1 monoclonal antibody, Sulph I, reacting with sulphatide (3'-sulphogalactosylceramide), was produced by immunizing Balb/c mice with that glycolipid coated on Salmonella minnesota bacterial membrane. Radioimmunodetection of the binding of the monoclonal antibody to structurally related glycolipids adsorbed to microtitre plates or chromatographed on thin-layer plates was used to determine its binding epitope. The antibody showed similar binding avidity to three sulphated glycolipids: sulphatide, sulpholactosylceramide and seminolipid. Lysosulphatide did bind the antibody, but, compared with sulphatide, 30 times more antigen was needed for half-maximal binding. Bis(sulphogangliotriosyl)ceramide and bis-sulphogangliotetraosylceramide did not bind the antibody. These results suggest that terminal galactose-3-O-sulphate and part of the hydrophobic region of the glycolipid are recognized by the Sulph I antibody.
一种与硫苷脂（3'-硫酸半乳糖神经酰胺）反应的IgG1单克隆抗体Sulph I，是通过用包被在明尼苏达沙门氏菌细菌膜上的那种糖脂免疫Balb/c小鼠产生的。利用放射免疫检测单克隆抗体与吸附在微量滴定板上或在薄层层析板上进行层析的结构相关糖脂的结合，来确定其结合表位。该抗体对三种硫酸化糖脂：硫苷脂、硫酸乳糖神经酰胺和半乳糖神经酰胺表现出相似的结合亲和力。溶血硫苷脂确实能结合该抗体，但与硫苷脂相比，达到半数最大结合所需的抗原量要多30倍。双（硫酸神经节三糖基）神经酰胺和双硫酸神经节四糖基神经酰胺不结合该抗体。这些结果表明，硫苷脂I抗体识别半乳糖-3-O-硫酸末端和糖脂疏水区域的一部分。
