Song Siyu, Liang Guangru Ben, Hormel Tristan T, Guo Yukun, Gao Min, Sivyer Benjamin, Campbell J Peter, Chen Siyu, Jian Yifan, Jia Yali
Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, USA.
Department of Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, USA.
Biomed Opt Express. 2025 May 19;16(6):2365-2375. doi: 10.1364/BOE.560539. eCollection 2025 Jun 1.
Microglial cells play a crucial role in retinal vascular and brain diseases through complex interactions with blood vessels and neurons. To image retinal structures, vasculature, and microglia, we developed a multimodal system integrating visible light optical coherence tomography (vis-OCT) and scanning laser ophthalmoscopy (SLO). Both subsystems achieve micron-scale resolutions and operate within the diffraction limit across a 34-degree field of view: theoretically, the OCT system offers an axial resolution of 2.12 μm and a transverse resolution of 8.78 μm, while the SLO system provides a transverse resolution of 7.1 μm. We validated the system performance using transgenic mice with fluorescent protein-labeled microglia, revealing detailed retinal microstructures, microvasculature, and individual microglia with distinguishable branches, confirmed by microscopy.
小胶质细胞通过与血管和神经元的复杂相互作用，在视网膜血管疾病和脑部疾病中发挥着关键作用。为了对视网膜结构、血管系统和小胶质细胞进行成像，我们开发了一种整合可见光光学相干断层扫描（vis-OCT）和扫描激光检眼镜（SLO）的多模态系统。两个子系统均实现了微米级分辨率，并在34度视野范围内的衍射极限内运行：理论上，OCT系统的轴向分辨率为2.12μm，横向分辨率为8.78μm，而SLO系统的横向分辨率为7.1μm。我们使用带有荧光蛋白标记小胶质细胞的转基因小鼠验证了系统性能，揭示了详细的视网膜微观结构、微血管系统以及具有可区分分支的单个小胶质细胞，这通过显微镜检查得到了证实。
