Huang Yun-Ju, Wu Hsin-Yu, Chang Wan-Ting, Lu Kuan-Hung, Lin Yu-En, Huang Huai-Syuan, Lu Yun-Sheng, Sheen Lee-Yan
Department of Biotechnology and Food Technology, Southern Taiwan University of Science and Technology, Tainan, Taiwan.
Institute of Food Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taiwan.
J Tradit Complement Med. 2024 May 16;15(4):414-422. doi: 10.1016/j.jtcme.2024.05.002. eCollection 2025 Jul.
(PC) is traditional Chinese medicine with sedative, diuretic, and tonic properties. rice (CMR) is a matrix used for the solid-state culture of C. militaris, which has been used for anti-angiogenetic and anti-inflammation effects. To investigate a formulation of combined and rice (PC-CMR) alleviates anxiety behaviours and depressive-like effects for the first time.
After five-week of stress, ats were induced by unpredictable chronic mild stress (UCMS) treated with PC-CMR formulation. Then, rats underwent various behavior tests, testing serum inflammatory mediators by enzyme-linked immunosorbent assays (ELISA), evaluated monoamines by high-performance liquid chromatography (HPLC) analysis, and protein expression by western blotting.
Treatment with doses of formulated PC-CMR reversed sucrose preference and increased amount of time spent on the open arms in rat behavioral tests. The formulated PC-CMR significantly reduced glutamate content and turnover rates of serotonin and dopamine in the prefrontal cortex of rats. In the inflammation-related pathway, PC-CMR decreased interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in serum and downregulated the expression of p38 mitogen-activated protein kinase (p38 MAPK), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the prefrontal cortex and signal transducer and activator of transcription 3 (STAT3), and indoleamine 2,3-dioxygenase (IDO) in the amygdala.
Our findings suggest that the formulated PC-CMR could have antidepressive and anxiolytic effects by modulating neurotransmitter levels and reducing inflammation.
（PC）是一种具有镇静、利尿和滋补特性的中药。 大米（CMR）是用于蛹虫草固态培养的基质，已被用于抗血管生成和抗炎作用。首次研究联合PC和大米（PC-CMR）配方缓解焦虑行为和抑郁样效应。
在五周应激后，通过用PC-CMR配方治疗的不可预测慢性轻度应激（UCMS）诱导大鼠产生应激。然后，大鼠进行各种行为测试，通过酶联免疫吸附测定（ELISA）检测血清炎症介质，通过高效液相色谱（HPLC）分析评估单胺，并通过蛋白质印迹法检测蛋白质表达。
在大鼠行为测试中，用配方PC-CMR剂量治疗可逆转蔗糖偏好并增加在开放臂上花费的时间量。配方PC-CMR显著降低大鼠前额叶皮质中谷氨酸含量以及血清素和多巴胺的周转率。在炎症相关途径中，PC-CMR降低血清中白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）水平，并下调前额叶皮质中p38丝裂原活化蛋白激酶（p38 MAPK）、活化B细胞核因子κB轻链增强子（NF-κB）以及杏仁核中信号转导和转录激活因子3（STAT3）和吲哚胺2,3-双加氧酶（IDO）的表达。
我们的研究结果表明，配方PC-CMR可能通过调节神经递质水平和减轻炎症而具有抗抑郁和抗焦虑作用。
