Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Province, Chengdu 611130, People's Republic of China.
Key Laboratory of Animal Disease-resistant Nutrition, Sichuan Province, Chengdu 611130, People's Republic of China.
J Anim Sci. 2024 Jan 3;102. doi: 10.1093/jas/skae194.
To explore the effects of cordyceps militaris (CM) on growth performance and intestinal epithelium functions, 180 weaned pigs were randomly assigned into 5 treatments with 6 replicate pens per treatment (6 pigs per pen). Pigs were fed with basal diet (control) or basal diet supplemented with 100, 200, 400, and 800 mg/kg CM. The trial lasted for 42 d, and pigs from the control and optimal-dose groups (based on growth performance) were picked for blood and tissue collection (n = 6). Results showed that CM elevated the average daily gain (ADG) and decreased the ratio of feed intake to gain (F:G) in the weaned pigs (P < 0.05). CM supplementation at 100 mg/kg improved the digestibilities of dry matter (DM), crude protein (CP), and gross energy (GE) (P < 0.05). CM not only increased the activities of superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) but also increased the concentration of interleukin-10 (IL-10) in serum (P < 0.05). The serum concentrations of malondialdehyde (MDA), d-lactate, and diamine oxidase (DAO) were reduced by CM (P < 0.05). Interestingly, CM elevated the villus height and the ratio of villus height to crypt depth in the duodenum and jejunum and increased the activities of duodenal sucrase and maltase (P < 0.05). Moreover, CM elevated the expression levels of tight-junction proteins ZO-1, claudin-1, and occluding, as well as critical functional genes such as the fatty acid transport protein (FATP1), cationic amino acid transporter 1 (CAT1), and NF-E2-related factor 2 (Nrf2) in the duodenum and jejunum (P < 0.05). Importantly, CM increased the concentrations of acetic acid and butyric acid, and elevated the abundances of Bacillus and Lactobacillus in the cecum and colon, respectively (P < 0.05). These results indicated potential benefits of CM in improving the growth of weaned pigs, and such effect may be tightly associated with improvement in antioxidant capacity and intestinal epithelium functions.
为了探究蛹虫草对生长性能和肠道上皮功能的影响，将 180 头断奶仔猪随机分成 5 个处理组，每个处理组设 6 个重复，每个重复 6 头猪。猪只饲喂基础日粮（对照组）或基础日粮添加 100、200、400 和 800mg/kg 蛹虫草。试验持续 42d，根据生长性能选择对照组和最佳剂量组（基于生长性能）的猪进行采血和组织采集（n=6）。结果表明，蛹虫草提高了断奶仔猪的平均日增重（ADG），降低了料重比（F:G）（P<0.05）。100mg/kg 蛹虫草添加量提高了干物质（DM）、粗蛋白（CP）和总能（GE）的消化率（P<0.05）。蛹虫草不仅提高了超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和过氧化氢酶（CAT）的活性，还提高了血清中白细胞介素-10（IL-10）的浓度（P<0.05）。血清中丙二醛（MDA）、D-乳酸和二胺氧化酶（DAO）的浓度因蛹虫草而降低（P<0.05）。有趣的是，蛹虫草提高了十二指肠和空肠的绒毛高度和绒毛高度与隐窝深度的比值，并提高了十二指肠蔗糖酶和麦芽糖酶的活性（P<0.05）。此外，蛹虫草提高了紧密连接蛋白 ZO-1、claudin-1 和闭合蛋白，以及脂肪酸转运蛋白（FATP1）、阳离子氨基酸转运蛋白 1（CAT1）和核因子 E2 相关因子 2（Nrf2）等关键功能基因在十二指肠和空肠中的表达水平（P<0.05）。重要的是，蛹虫草增加了盲肠和结肠中乙酸和丁酸的浓度，并分别提高了盲肠和结肠中芽孢杆菌和乳酸杆菌的丰度（P<0.05）。这些结果表明，蛹虫草在提高断奶仔猪生长性能方面具有潜在的益处，这种作用可能与抗氧化能力和肠道上皮功能的改善密切相关。
