Wang Lin, Dong Qiong, Zheng Shaojie, Li Xingze, Zeng Huiping, Chen Yu, Shi Shunrou
College of Forestry, Southwest Forestry University, Kunming, China.
Key Laboratory of Southwest Biodiversity Conservation, State Forestry and Grassland Administration, Kunming, China.
Front Microbiol. 2025 Jul 3;16:1613698. doi: 10.3389/fmicb.2025.1613698. eCollection 2025.
To explore the differences in soil bacterial and fungal community characteristics under forests with different rainfall amounts, natural forests in Guangnan County (GN), Jianshui County (JS), and Yuanjiang County (YJ) of Yunnan Province, China, were selected as the research objects.
Three 20 × 20 standard plots were set up for each forest stand. The antioxidant system of seedlings in different plots was determined, and high-throughput sequencing technology was used to analyze soil bacteria and fungi.
The results showed that during the growth process of , the activities of SOD and POD, as well as the content of Pro, were highest in GN, while the content of SP was highest in the YJ. Significant differences were found in the -diversity of soil bacterial and fungal communities among regions ( < 0.05). Specifically, for soil bacteria, the Shannon, Chao1, and ACE (Abundance-based Coverage Estimator) indices were highest in GN and lowest in JS; for fungi, the Chao1 and ACE indices were highest in YJ and lowest in JS. The dominant phyla of soil bacterial communities in GN and JS regions were , and , whereas in the YJ region were , and . The dominant phyla of soil fungal communities across all regions were and . Through the comparison of Tax4Fun and FUNGuild functions, it was found that more than 80% of the main functions of soil bacteria in various regions are related to metabolism. Soil fungi were primarily of the nutritional functional types, and the main functional group was fungi. In addition, changes in rainfall had minimal effect on the functional abundance of soil bacterial communities but had a more significant impact on the functional abundance of fungal communities.
In summary, the soil bacterial community diversity and richness index of in the GN region is the highest, and the bacterial phylum related to plant growth and fungi account for the highest proportion. This study elucidated the response mechanism of to changes in rainfall from a microbial perspective, providing a theoretical basis for their growth being regulated by rainfall.
为探究不同降雨量地区森林土壤细菌和真菌群落特征的差异，选取中国云南省广南县（GN）、建水县（JS）和元江县（YJ）的天然林作为研究对象。
为每个林分设置3个20×20的标准样地。测定不同样地中幼苗的抗氧化系统，并采用高通量测序技术分析土壤细菌和真菌。
结果表明，在[具体植物名称未给出]生长过程中，超氧化物歧化酶（SOD）和过氧化物酶（POD）活性以及脯氨酸（Pro）含量在GN最高，而可溶性蛋白（SP）含量在YJ最高。各地区土壤细菌和真菌群落的α多样性存在显著差异（P<0.05）。具体而言，对于土壤细菌，香农指数、Chao1指数和基于丰度的覆盖度估计器（ACE）指数在GN最高，在JS最低；对于真菌，Chao1指数和ACE指数在YJ最高，在JS最低。GN和JS地区土壤细菌群落的优势菌门为[具体菌门名称未给出]，而YJ地区为[具体菌门名称未给出]。所有地区土壤真菌群落的优势菌门为[具体菌门名称未给出]和[具体菌门名称未给出]。通过Tax4Fun和FUNGuild功能比较发现，各地区土壤细菌的主要功能中80%以上与代谢有关。土壤真菌主要为[具体营养功能类型未给出]营养功能类型，主要功能组为[具体真菌类型未给出]真菌。此外，降雨变化对土壤细菌群落功能丰度的影响最小，但对真菌群落功能丰度的影响更为显著。
综上所述，GN地区[具体植物名称未给出]的土壤细菌群落多样性和丰富度指数最高，与植物生长相关的细菌门和[具体真菌类型未给出]真菌所占比例最高。本研究从微生物角度阐明了[具体植物名称未给出]对降雨变化的响应机制，为其生长受降雨调控提供了理论依据。
