Iijima M, Kubota K, Matsuoka Y, Uruno T, Satoh S, Ueki M, Matsui S
J Pharmacobiodyn. 1985 Jul;8(7):549-56. doi: 10.1248/bpb1978.8.549.
The characteristic of the antinociceptive effect of a tetrapeptide, Asn-Ala-Gly-Ala (NAGA), was examined. Intracisternal (i.cist.) administration of NAGA to mice produced dose-dependent and long-lasting antinociceptive effect as evaluated by the hot plate and tail flick methods. NAGA-induced antinociception was reversed by naloxone (2 mg/kg), reserpine (2 mg/kg X 2 d) and alpha-methyl-p-tyrosine (100 mg/kg). NAGA (10 microM) did not significantly affect the actions of opioid peptides on the electrically evoked twitches of the myenteric plexus-longitudinal muscle strips from the guinea-pig esophagus and strips of the mouse vas deferens. NAGA-induced antinociception was scarcely affected by p-chlorophenylalanine (PCPA, 300 mg/kg X 2 d) and methysergide (2 mg/kg). From the above results, it was suggested that the antinociceptive effect of NAGA may involve the participation of endogenous opioid peptides and endogenous catecholamines.
对一种四肽天冬酰胺-丙氨酸-甘氨酸-丙氨酸（NAGA）的抗伤害感受作用特性进行了研究。通过热板法和甩尾法评估，向小鼠脑池内注射NAGA产生了剂量依赖性和持久的抗伤害感受作用。纳洛酮（2毫克/千克）、利血平（2毫克/千克×2天）和α-甲基-对-酪氨酸（100毫克/千克）可逆转NAGA诱导的抗伤害感受。NAGA（10微摩尔）对阿片肽对豚鼠食管肌间神经丛-纵肌条和小鼠输精管条电诱发抽搐的作用无显著影响。对氯苯丙氨酸（PCPA，300毫克/千克×2天）和麦角新碱（2毫克/千克）对NAGA诱导的抗伤害感受几乎没有影响。根据上述结果，提示NAGA的抗伤害感受作用可能涉及内源性阿片肽和内源性儿茶酚胺的参与。
