He C, Song C Y, Wei Y M, You Z D, Shi P T
Department of Biochemistry, Second Military Medical University, Shanghai, China.
Zhongguo Yao Li Xue Bao. 1994 Nov;15(6):497-500.
The antinociceptive effect of intracerebroventricular injection (icv) of Asn-Ala-Gly-Ala (NAGA), a partial sequence of beta-lipotropin, was studied in rats. The potassium iontophoresis-induced tail flick was used to measure the pain threshold. The antinociceptive effect of NAGA, which was dose-dependent (icv, 0.03-0.24 mumol/rat) and long-lasting (90 min), was reversed by naloxone (icv, 0.26 mg.kg-1) and inhibited by anti-MEK serum (titre: 1:5000, 5 microliters) or anti-LEK serum (titre: 1:5000, 5 microliters). NAGA-induced antinociception was scarcely affected by anti-beta-EP serum (titre: 1:30,000, 5 microliters) or anti-Dyn A1-13 serum (titre: 1:30,000, 5 microliters). It was suggested that the antinociceptive effect of NAGA may be associated with the release of met-enkephalin and leu-enkephalin in rat brain.
在大鼠中研究了脑室内注射（icv）β-促脂素的部分序列天冬酰胺-丙氨酸-甘氨酸-丙氨酸（NAGA）的抗伤害感受作用。采用钾离子透入法诱导甩尾来测量痛阈。NAGA的抗伤害感受作用呈剂量依赖性（icv，0.03 - 0.24 μmol/大鼠）且持续时间长（90分钟），可被纳洛酮（icv，0.26 mg·kg⁻¹）逆转，并被抗MEK血清（效价：1:5000，5微升）或抗LEK血清（效价：1:5000，5微升）抑制。NAGA诱导的抗伤害感受几乎不受抗β - EP血清（效价：1:30,000，5微升）或抗强啡肽A1 - 13血清（效价：1:30,000，5微升）的影响。提示NAGA的抗伤害感受作用可能与大鼠脑内甲硫氨酸脑啡肽和亮氨酸脑啡肽的释放有关。
