Covaci Antoanela Magdalena, Andrei Mihai, Dinca Ioana, Ciocan Lucian Toma, Matei Madalina Nicoleta, Didilescu Andreea Cristiana
Department of Embryology and Microbiology, Faculty of Dentistry, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania.
Department of Dental Medicine, Faculty of Medicine and Pharmacy, Dunarea de Jos University of Galati, 800010 Galati, Romania.
Curr Health Sci J. 2025 Jan-Mar;51(1):96-104. doi: 10.12865/CHSJ.51.01.10. Epub 2025 Mar 31.
Pulp capping is a vital pulp therapy that aims to prolong the life of a tooth by protecting it after pulp exposure occurred. Pulp capping biomaterials are intended to induce odontoblasts to deposit a natural tertiary dentin barrier to protect the pulp-dentin complex. Two pulp capping agents with calcium silicates in their composition, but with different mechanisms of the setting reaction were tested in vivo with the main objective of comparing their effect on the pulp-dentin complex. The specific aim was to evaluate the preservation of pulp vitality following in vivo direct and indirect pulp capping on eight human third molars. TheraCal LC, a light-cured calcium silicate-based material, was tested both by direct and indirect pulp capping, while the mineral trioxide aggregate (MTA) cement was tested by direct pulp capping. The molars were assessed by micro-computed tomography (micro-CT) and by light microscopy and stereo-microscopy following histological processing of the teeth. Dental pulp vitality testing was performed before tooth extraction. Inflammatory pulp status was performed on light microscopy images and it was investigated the presence of inflammatory infiltrate, edema, vascular congestion and pulp necrosis. Following pulp capping, the MTA cement showed more favorable results, generating the formation of complete or incomplete dentin bridges in all treated teeth, while TheraCal LC induced the formation of dentin bridges in only two teeth. Tooth vitality was preserved in all tested teeth. In conclusion, both materials stimulated neodentinogenesis, with the MTA cement being more effective and presenting a much more favorable biological pulpal response.
盖髓术是一种重要的牙髓治疗方法，其目的是在牙髓暴露后通过保护牙齿来延长其寿命。盖髓生物材料旨在诱导成牙本质细胞沉积天然的第三期牙本质屏障，以保护牙髓-牙本质复合体。测试了两种成分中含有硅酸钙但凝固反应机制不同的盖髓剂，其主要目的是比较它们对牙髓-牙本质复合体的影响。具体目标是评估在八颗人类第三磨牙上进行体内直接和间接盖髓后牙髓活力的保存情况。TheraCal LC是一种光固化的硅酸钙基材料，通过直接和间接盖髓进行测试，而矿物三氧化物凝聚体（MTA）水泥则通过直接盖髓进行测试。在牙齿进行组织学处理后，通过微计算机断层扫描（micro-CT）、光学显微镜和立体显微镜对磨牙进行评估。在拔牙前进行牙髓活力测试。在光学显微镜图像上进行炎性牙髓状态评估，并研究炎性浸润、水肿、血管充血和牙髓坏死的存在情况。盖髓后，MTA水泥显示出更有利的结果，在所有治疗的牙齿中均产生了完全或不完全的牙本质桥形成，而TheraCal LC仅在两颗牙齿中诱导形成了牙本质桥。所有测试牙齿的牙髓活力均得以保存。总之，两种材料均刺激了新牙本质形成，MTA水泥更有效，并且呈现出更有利的牙髓生物学反应。
