Adıgüzel Mehmet, Ahmetoğlu Fuat, Eldeniz Ayçe Ünverdi, Tekin Mehmet Gökhan, Göğebakan Bülent
Department of Endodontics, Faculty of Dentistry, Mustafa Kemal University, Hatay, Turkey.
Private Practice, Malatya, Turkey.
J Dent Res Dent Clin Dent Prospects. 2019 Fall;13(4):241-246. doi: 10.15171/joddd.2019.037.
This study aimed to compare the in vitro cytotoxicity of Theracal LC, BiodentineTM, iRoot BP Plus, and MTA Angelus on human pulp fibroblasts (HPF). Fifteen discs from each calcium silicate-based material were prepared in sterile Teflon molds. After setting, the fabricated discs were eluated with a culture medium for 24 h. HPF cells were plated onto 24-well plates at 5×10 cells/well, and the cells were exposed to the material eluates. The cell viability was evaluated with MTT assay at three different times (24, 48, and 72 h). Data were statistically analyzed. The apoptotic/necrotic status of HPF cells exposed to material eluates was determined by flow cytometry. The differences between the effects of Theracal LC, Biodentine, MTA Angelus, and iRoot BP Plus on HPF cells were found to be statistically significant (P<0.05). Theracal LC was found to be more cytotoxic considering other vital pulp capping materials at 24- (28.3%), 48- (44.9%), and 72-hour (49.2%) intervals. On the other hand, Biodentine showed the least cytotoxic effects (97.1%, 130.0%, and 103.7%, respectively) According to flow cytometry results, Theracal LC material increased apoptosis/necrosis ratios compared to the other materials. Based on the results of the present study, BiodentineTM, MTA Angelus, and iRoot BP Plus can be classified as biocompatible materials in vital endodontic treatments. However, the Theracal LC materials should be used carefully due to their cytotoxic effects.
本研究旨在比较Theracal LC、BiodentineTM、iRoot BP Plus和MTA Angelus对人牙髓成纤维细胞（HPF）的体外细胞毒性。在无菌聚四氟乙烯模具中制备每种硅酸钙基材料的15个圆盘。凝固后，将制成的圆盘用培养基洗脱24小时。将HPF细胞以5×10个细胞/孔接种到24孔板中，并将细胞暴露于材料洗脱液中。在三个不同时间点（24、48和72小时）用MTT法评估细胞活力。对数据进行统计学分析。通过流式细胞术确定暴露于材料洗脱液的HPF细胞的凋亡/坏死状态。发现Theracal LC、Biodentine、MTA Angelus和iRoot BP Plus对HPF细胞的作用之间存在统计学显著差异（P<0.05）。发现在24小时（28.3%）、48小时（44.9%）和72小时（49.2%）的时间间隔内，与其他重要牙髓盖髓材料相比，Theracal LC具有更高的细胞毒性。另一方面，Biodentine显示出最小的细胞毒性作用（分别为97.1%、130.0%和103.7%）。根据流式细胞术结果，与其他材料相比，Theracal LC材料增加了凋亡/坏死率。根据本研究结果，BiodentineTM、MTA Angelus和iRoot BP Plus在重要牙髓治疗中可归类为生物相容性材料。然而，由于Theracal LC材料具有细胞毒性作用，应谨慎使用。
