Cinar Topcu Kübra, Altun Güven Sara, Cakir Özlem, Anlar Pınar, Durul Melekber Sulusoglu, Ay Mizgin, Alyafei Mohammed, Hayat Faisal, Ercisli Sezai
Aydıntepe Vocational School, Department of Food Processing Bayburt University Bayburt Turkey.
Faculty of Engineering, Department of Computer Engineering Tarsus University Mersin Turkey.
Food Sci Nutr. 2025 Jul 17;13(7):e70610. doi: 10.1002/fsn3.70610. eCollection 2025 Jul.
This study aimed to optimize the ethanol extraction conditions of sun-dried apricot ( L.) and evaluate the antioxidant capacity, phenolic, and flavonoid contents of the obtained extracts. Response surface methodology (RSM) was applied to determine the optimal extraction conditions, which were identified as 60°C temperature, 34% ultrasonic power, 46 min sonication time, and a 4 g/mL solid-liquid ratio. Under these conditions, the extract exhibited a total phenolic content (TPC) of 4.20 mg GAE/g, a total flavonoid content (TFC) of 7.09 mg QE/g, a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity of 1.37 mg TE/g, a ferric reducing antioxidant power (FRAP) value of 9.12 mg TE/g, and a thiobarbituric acid reactive substances (TBARS) level of 1.69 mg MDA/g. The artificial neural network (ANN) model provided highly accurate predictions for these parameters. Additionally, cell culture experiments demonstrated that the extract exerted a dose-dependent cytotoxic effect on HCT116 colon cancer cells, significantly reducing their viability. These findings highlight the potential of sun-dried apricot extracts as natural antioxidants with possible applications in the functional food and pharmaceutical industries.
本研究旨在优化杏干的乙醇提取条件，并评估所得提取物的抗氧化能力、酚类和黄酮类含量。采用响应面法（RSM）确定最佳提取条件，确定为60°C温度、34%超声功率、46分钟超声时间和4 g/mL固液比。在这些条件下，提取物的总酚含量（TPC）为4.20 mg GAE/g，总黄酮含量（TFC）为7.09 mg QE/g，2,2-二苯基-1-苦基肼（DPPH）自由基清除能力为1.37 mg TE/g，铁还原抗氧化能力（FRAP）值为9.12 mg TE/g，硫代巴比妥酸反应物质（TBARS）水平为1.69 mg MDA/g。人工神经网络（ANN）模型对这些参数提供了高度准确的预测。此外，细胞培养实验表明，该提取物对HCT116结肠癌细胞具有剂量依赖性细胞毒性作用，显著降低了它们的活力。这些发现突出了杏干提取物作为天然抗氧化剂在功能性食品和制药行业中可能应用的潜力。
