Keleştemur Ünzile, Balcioğlu Sevgi, Ateş Burhan
Faculty of Health Sciences, Department of Occupational Health and Safety, Muş Alparslan University, Muş, Turkey.
Department of Medicinal Laboratory, Sakarya University of Applied Sciences, Sakarya, Turkey.
J Food Sci. 2025 Jan;90(1):e17595. doi: 10.1111/1750-3841.17595. Epub 2024 Dec 14.
In recent years, the role of functional foods in addressing various health issues, including cancer, has gained significant attention. Among these, the cytotoxic and apoptotic properties of Malatya apricots hold particular interest for their potential therapeutic benefits. This study focused on exploring the effects of methanol and acetone extracts from four popular Malatya apricot varieties-Hacıhaliloğlu, Hasanbey, Kabaaşı, and Soğancı-on cancer cell lines. The extracts were derived from apricots in their raw, ripe, and dried form, which can be consumed in three ways, and were tested for their cytotoxic and apoptotic activities against MCF-7 and Caco-2 cell lines using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, wound scratch, and acridine orange/ethidium bromide (AO/EB) staining tests. Additionally, we assessed the total phenolic and flavonoid contents of the extracts. Our findings revealed that the acetone extracts of dried and raw Kabaaşı apricots exhibited the lowest IC values against the MCF-7 cell line over 24 and 48 h, with values of 3.9 and 0.5 mg/mL, respectively. Similarly, in the Caco-2 cell line, the dried Kabaaşı apricot extracts showed the lowest ICvalues of 3.59 and 1.95 mg/mL for the same time frames. In the wound scratch assay, utilizing apricot extracts at their lowest IC values revealed significant differences in cell migration inhibition between treated and control groups. For Caco-2 cells, the control group's wound closure was around 70%, whereas apricot extract treatment resulted in 10%-30% opening after 24 h, indicating a strong antiproliferative effect. Similarly, in MCF-7 cells, the control group showed approximately 80% wound closure, in contrast to the 0%-12% opening observed in cells treated with apricot extract, further emphasizing the extracts' potent inhibitory impact on cell proliferation. Additionally, morphological evidence of cell death and apoptosis was observed in the images obtained through the AO/EB staining method, conducted to showcase the cytotoxic and apoptotic effects of the apricot extract. Apricot extracts consistently demonstrated strong cytotoxic and apoptotic effects on both Caco-2 and MCF-7 cell lines, underscoring Malatya apricots' potential as a functional food component with promising therapeutic properties against cancer.
近年来，功能性食品在解决包括癌症在内的各种健康问题中所起的作用受到了广泛关注。其中，马拉蒂亚杏子的细胞毒性和凋亡特性因其潜在的治疗益处而备受关注。本研究聚焦于探究四种常见的马拉蒂亚杏子品种——哈奇哈利洛卢、哈桑贝伊、卡巴阿şı和索甘cı——的甲醇提取物和丙酮提取物对癌细胞系的影响。这些提取物分别来自生的、成熟的和干燥的杏子，有三种食用方式，并使用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-四氮唑溴盐、伤口划痕和吖啶橙/溴化乙锭（AO/EB）染色试验，测试了它们对MCF-7和Caco-2细胞系的细胞毒性和凋亡活性。此外，我们还评估了提取物中的总酚和黄酮含量。我们的研究结果显示，干燥的和生的卡巴阿şı杏子的丙酮提取物在24小时和48小时内对MCF-7细胞系的IC值最低，分别为3.9和0.5毫克/毫升。同样，在Caco-2细胞系中，干燥的卡巴阿şı杏子提取物在相同时间范围内的IC值最低，分别为3.59和1.95毫克/毫升。在伤口划痕试验中，使用最低IC值的杏子提取物显示，处理组和对照组之间在细胞迁移抑制方面存在显著差异。对于Caco-2细胞，对照组的伤口闭合率约为70%，而杏子提取物处理后24小时伤口开口率为10%-30%，表明具有很强的抗增殖作用。同样，在MCF-7细胞中，对照组的伤口闭合率约为80%，相比之下，用杏子提取物处理的细胞伤口开口率为0%-12%，进一步强调了提取物对细胞增殖的强大抑制作用。此外，通过AO/EB染色法获得的图像中观察到了细胞死亡和凋亡的形态学证据，以展示杏子提取物的细胞毒性和凋亡作用。杏子提取物对Caco-2和MCF-7细胞系始终表现出很强的细胞毒性和凋亡作用，突出了马拉蒂亚杏子作为一种功能性食品成分对癌症具有潜在治疗特性的潜力。
