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猪球虫德氏艾美耳球虫(Eimeria debliecki Douwes,1921)在哺乳动物细胞培养中的发育

Development of the swine coccidium Eimeria debliecki Douwes, 1921 in mammalian cell cultures.

作者信息

Lindsay D S, Blagburn B L, Current W L, Ernst J V

出版信息

J Protozool. 1985 Nov;32(4):669-71. doi: 10.1111/j.1550-7408.1985.tb03099.x.

DOI:10.1111/j.1550-7408.1985.tb03099.x
PMID:4067880
Abstract

Sporozoites of Eimeria debliecki entered human fetal lung and porcine kidney cells grown in cultures and underwent one merogenous cycle, terminating in the production of second-generation trophozoites. Sporozoites were intracellular 1 h post-inoculation (PI) and developed into sporozoite-shaped meronts at 40 h PI. These meronts, one of which was motile, had from two to ten nuclei. Sporozoite-shaped meronts then developed into elongate or spheroidal meronts with 10 to 24 nuclei by two days PI. Ten to 26 first-generation merozoites were formed by budding from the meront surface. Mature first-generation merozoites were most numerous three days PI. Most meronts had ruptured and released nonmotile merozoites into the culture medium by four days PI. Merozoites that were not released became rounded and developed into second-generation trophozoites. Refractile bodies were present in all developmental stages. No further development was observed five through eight days PI.

摘要

德氏艾美耳球虫的子孢子进入培养的人胎儿肺细胞和猪肾细胞,并经历了一个裂殖生殖周期,最终产生第二代滋养体。接种后1小时(PI)子孢子进入细胞内,接种后40小时发育成子孢子形状的裂殖体。这些裂殖体,其中一个是可运动的,有2到10个细胞核。到接种后两天,子孢子形状的裂殖体发育成具有10到24个细胞核的细长或球形裂殖体。通过从裂殖体表面出芽形成10到26个第一代裂殖子。接种后三天成熟的第一代裂殖子数量最多。到接种后四天,大多数裂殖体破裂,将不活动的裂殖子释放到培养基中。未释放的裂殖子变圆并发育成第二代滋养体。在所有发育阶段都有折光体存在。接种后5至8天未观察到进一步发育。

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